Issue 1

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 1, March 2015, Pages 5–8

ISSN XXXX-XXXX (print version) ISSN ISSN 2411-0388 (online version)


Obukhovska O. V., Stegniy B. T., Glebova K. V., Shutchenko P. O., Medved K. O.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkov, Ukraine, e-mail:

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Citation for print version: Obukhovska, O. V., Stegniy, B. T., Glebova, K. V., Shutchenko, P. O. and Medved, K. O. (2015) ‘The macrophages accumulation in chickens vaccinated against avian mycoplasmosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(1), pp. 5–8.

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Citation for online version: Obukhovska, O. V., Stegniy, B. T., Glebova, K. V., Shutchenko, P. O. and Medved, K. O. (2015) ‘The macrophages accumulation in chickens vaccinated against avian mycoplasmosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(1), pp. 5–8. Available at:

Summary. The goal of our investigations was determination of the dynamics and intensity of macrophages accumulation in the immunocompetent organs of chickens vaccinated against Avian mycoplasmosis.

For creation of experimental series vaccines we have applied two technologies. In the first series as an antigenic bases used formaldehyde inactivated bacterin of production strain Mycoplasma gallisepticum VK (VB); in the second series – ultrasound disintegrated bacterial mass of cells of the same strain (VS). Experiments were carried out on chickens. Birds of first experimental group (n = 30) were immunized intramuscularly twice at an interval of 30 days by vaccine VB (VB group). Birds of second experimental group (n = 30) were immunized at the same scheme by vaccine VS (VS group). Birds of control group (n = 30) was not vaccinated.

On the 7th, 10th, 14th and 21st days after the second injection of vaccines 5 individuals from each group were euthanized; from birds were taken lungs, trachea, spleen and caecum tonsil. Preparations were stained by immuno-histochemistry method using labeled streptavidin-biotin. Presence and percentage of cell populations macrophages into organ samples account in the process of smear microscopy.

Macrophages are actually the first link of cellular immunity. After immunization the activation occurs in a relatively short time after injection of immunizing substance. It is this process we observed in the study of the internal organs of immunized chickens.

It was found that injection of inactivated vaccines in chickens promoted stimulation for primary link of cellular immunity. The population of macrophages increased rapidly during the first 10 days after the second injection of both vaccines. The highest value of this indicator was recorded in the spleen and lungs of birds (24.125 % and 22.280 % in the VB group; 21.010 % and 20.333 % in the VS group). Over the next 11 days, their number gradually decreased and on 21st day almost reached the level of the Control group. However, in VB group, this process was more intense, as evidenced by high values recorded during the study.

Keywords: inactivated vaccine, avian mycoplasmosis, macrophages


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