Issue 1
Journal
for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue
1, March 2015, Pages 5–8
ISSN XXXX-XXXX (print version) ISSN ISSN 2411-0388 (online version)
THE MACROPHAGES ACCUMULATION IN CHICKENS VACCINATED
AGAINST AVIAN MYCOPLASMOSIS
Obukhovska O.
V., Stegniy B. T., Glebova
K. V., Shutchenko P. O., Medved
K. O.
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PDF (print version)
Citation for print version: Obukhovska, O. V., Stegniy, B. T., Glebova, K. V., Shutchenko, P. O. and Medved, K.
O. (2015) ‘The macrophages accumulation in chickens vaccinated against
avian mycoplasmosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(1), pp. 5–8.
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PDF (online version)
Citation for online version: Obukhovska, O. V.,
Stegniy, B. T., Glebova, K.
V., Shutchenko, P. O. and Medved,
K. O. (2015) ‘The macrophages accumulation in chickens vaccinated against
avian mycoplasmosis’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(1), pp. 5–8. Available at:
http://jvmbbs.kharkov.ua/archive/2015/volume1/issue1/jvmbbso2015011_005-008.pdf
Summary. The goal of our
investigations was determination of the dynamics and intensity of macrophages
accumulation in the immunocompetent organs of chickens
vaccinated against Avian mycoplasmosis.
For creation of experimental series
vaccines we have applied two technologies. In the first series as an antigenic
bases used formaldehyde inactivated bacterin of
production strain Mycoplasma gallisepticum
VK (VB); in the second series – ultrasound
disintegrated bacterial mass of cells of the same strain (VS). Experiments were
carried out on chickens. Birds of first experimental group (n = 30) were
immunized intramuscularly twice at an interval of 30 days by vaccine VB (VB
group). Birds of second experimental group (n = 30) were immunized at the same
scheme by vaccine VS (VS group). Birds of control group (n = 30) was not
vaccinated.
On the 7th, 10th,
14th and 21st days after the second injection of vaccines
5 individuals from each group were euthanized; from birds were taken lungs,
trachea, spleen and caecum tonsil. Preparations were
stained by immuno-histochemistry method using labeled
streptavidin-biotin. Presence and percentage of cell
populations macrophages into organ samples account in the process of smear
microscopy.
Macrophages are actually the first
link of cellular immunity. After immunization the activation occurs in a
relatively short time after injection of immunizing substance. It is this process
we observed in the study of the internal organs of immunized chickens.
It was found that injection of
inactivated vaccines in chickens promoted stimulation for primary link of
cellular immunity. The population of macrophages increased rapidly during the
first 10 days after the second injection of both vaccines. The highest value of
this indicator was recorded in the spleen and lungs of birds (24.125 % and
22.280 % in the VB group; 21.010 % and 20.333 % in the VS group). Over the next
11 days, their number gradually decreased and on 21st day almost
reached the level of the Control group. However, in VB group, this process was
more intense, as evidenced by high values recorded during the study.
Keywords: inactivated vaccine, avian mycoplasmosis, macrophages
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