Issue 2

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 2, June 2015, Pages 25–31

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STuDY Of THE GENETIC VARIABILITY Of THE PORCINE CIRCOVIRUS TyPe 2 IN UkRAINe

Gerilovych A. P., Stegniy B. T., Rudova N. G., Buzun A. I.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: antger2011@gmail.com, antger@vet.kharkov.ua

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Citation for print version: Gerilovych A. P., Stegniy B. T., Rudova N. G. and Buzun A. I. (2015) ‘Study of the genetic variability of the porcine circovirus type 2 in Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(2), pp. 25–31.

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Citation for online version: Gerilovych A. P., Stegniy B. T., Rudova N. G. and Buzun A. I. (2015) ‘Study of the genetic variability of the porcine circovirus type 2 in Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(2), pp. 25–31. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue2/oJVMBBS_2015012_025-031.pdf

Summary. Porcine circoviral infection is the economically significant and contagious porcine disease, affecting of the animals from different ages and causing multiple syndromes associated with immunodeficiency. The aim of this work consisted in the study of genetic polymorphism of the Porcine circovirus type 2 strains circulating in different farms in Ukraine to determine their genotype.

Study was performed by the molecular (PCR, sequencing) and bioinformatical (multiple alignment, phylogenetic study) methods. Samples were collected using basic methods from the productive pig farms in Eastern Ukraine. Rep gene 421 bp region has been used as the matrix for sequencing and phylogenetic analysis.

The 6 characterized isolates were related to 1 and 2 genotypes of PCV-2. They demonstrated different levels of variability in the comparison with strains from European, Asian and American origin, allocated in different countries.

The study of the PCV-2 genetic differences in 421 bp rep gene loci demonstrated relation of isolates to American-origin contaminants of the veterinary preparations — genotype 2, and other field strains were related to European progenitors of genotype 1 (70–93% homology).

Keywords: Porcine circovirus type 2, genotype, PCR, phylogenetic analysis

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