Issue 2

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 2, June 2015, Pages 25–31

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Gerilovych A. P., Stegniy B. T., Rudova N. G., Buzun A. I.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail:,

Download PDF (print version)

Citation for print version: Gerilovych A. P., Stegniy B. T., Rudova N. G. and Buzun A. I. (2015) ‘Study of the genetic variability of the porcine circovirus type 2 in Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(2), pp. 25–31.

Download PDF (online version)

Citation for online version: Gerilovych A. P., Stegniy B. T., Rudova N. G. and Buzun A. I. (2015) ‘Study of the genetic variability of the porcine circovirus type 2 in Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(2), pp. 25–31. Available at:

Summary. Porcine circoviral infection is the economically significant and contagious porcine disease, affecting of the animals from different ages and causing multiple syndromes associated with immunodeficiency. The aim of this work consisted in the study of genetic polymorphism of the Porcine circovirus type 2 strains circulating in different farms in Ukraine to determine their genotype.

Study was performed by the molecular (PCR, sequencing) and bioinformatical (multiple alignment, phylogenetic study) methods. Samples were collected using basic methods from the productive pig farms in Eastern Ukraine. Rep gene 421 bp region has been used as the matrix for sequencing and phylogenetic analysis.

The 6 characterized isolates were related to 1 and 2 genotypes of PCV-2. They demonstrated different levels of variability in the comparison with strains from European, Asian and American origin, allocated in different countries.

The study of the PCV-2 genetic differences in 421 bp rep gene loci demonstrated relation of isolates to American-origin contaminants of the veterinary preparations — genotype 2, and other field strains were related to European progenitors of genotype 1 (70–93% homology).

Keywords: Porcine circovirus type 2, genotype, PCR, phylogenetic analysis


Allan, G. M., McNeilly, F., Meehan, B. M., Kennedy, S., Mackie, D. P., Ellis, J. A., Clark, E. G., Espuna, E., Saubi, N., Riera, P., Bøtner, A. and Charreyre, C. E. (1999) ‘Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland’, Veterinary Microbiology, 66(2), pp. 115–123. doi:

Allan, G. M., Caprioli, A., McNair, I., Lagan-Tregaskis, P., Ellis, J., Krakowka, S., McKillen, J., Ostanello, F. and McNeilly, F. (2007) ‘Porcine circovirus 2 replication in colostrum-deprived piglets following experimental infection and immune stimulation using a modified live vaccine against porcine respiratory and reproductive syndrome virus’, Zoonoses and Public Health, 54(5), pp. 214–222. doi:

Anon. (2005) Proceedings of the conference ‘Animal circoviruses and associated diseases’. Belfast, UK, 11–13 September 2005

Anon. (2007) Proceedings of the 5th international symposium on emerging and re-emerging pig diseases. Krakow, Poland, 24–27 June 2007

Blotska, O. F. (2008) ‘Porcine circoviral infection’ [Tsyrkovirusna infektsiia svynei]. Veterinary medicine of Ukraine [Veterynarna medytsyna Ukrainy], 12, pp. 21–22. [in Ukrainian]

Choi, C., Chae, C. and Clark, E. G. (2000) ‘Porcine postweaning multisystemic wasting syndrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain reaction’, Journal of Veterinary Diagnostic Investigation, 12(2), pp. 151–153. doi:

De Boisseson, C., Béven, V, Bigarré, L., Thiéry, R., Rose, N., Eveno, E., Madec, F. and Jestin, A. (2004) ‘Molecular characterization of porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs’, Journal of General Virology, 85(2), pp. 293–304. doi:

Fort, M., Olvera, A., Sibila, M., Segalés, J. and Mateu, E. (2007) ‘Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs’, Veterinary Microbiology, 125(3-4), pp. 244–255. doi:

Giammarioli, M., Pellegrini, C., Casciari, C. and De Mia, G. M. (2007) ‘Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus’, Veterinary Research Communications, 32(3), pp. 255–262. doi:

Kim, J. and Chae, C. (2001) ‘Differentiation of porcine circovirus 1 and 2 in formalin-fixed, paraffin-wax-embedded tissues from pigs with postweaning multisystemic wasting syndrome by in-situ hybridisation’, Research in Veterinary Science, 70(3), pp. 265–269. doi:

Kovalenko, A. M., Guz’, S. A., Anichin, R. Yu., Bolotin, V. I. and Peisak, Z. (2005) ‘Problems of porcine circoviral infection’ [Problemy tsirkovirusnoy infektsii sviney]. Veterinary Medicine [Veterynarna medytsyna], 85(1), pp. 523–528. [in Russian]

Kritas, S. K., Alexopoulos, C., Kyriakis, C. S., Tzika, E. and Kyriakis, S. C. (2007) ‘Performance of fattening pigs in a farm infected with both porcine reproductive and respiratory syndrome (PRRS) virus and porcine circovirus type 2 following sow and piglet vaccination with an attenuated PRRS vaccine’, Journal of Veterinary Medicine Series A, 54(6), pp. 287–291. doi:

Lee, C.‑S., Moon, H.‑J., Yang, J.‑S., Park, S.‑J., Song, D.‑S., Kang, B.‑K. and Park, B.‑K. (2007) ‘Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs’, Journal of Virological Methods, 139(1), pp. 39–43. doi:

Orlyankin, B. G., Aliper, T. I. and Nepoklonov, Ye. A. (2002) ‘Modern recognition of the porcine circoviruses’ [Sovremennye predstavleniya o tsirkovirusakh sviney]. Agricultural Biology. Animal Biology [Sel'skokhozyaystvennaya biologiya. Biologiya zhivotnykh], 6, pp. 29–37. [in Russian]

Shang, S.‑B., Li, Y.‑F., Guo, J.‑Q., Wang, Z.‑T., Chen, Q.‑X., Shen, H.‑G. and Zhou, J.‑Y. (2008) ‘Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2’, Research in Veterinary Science, 84(1), pp. 150–157. doi:

Shkayeva, M. A., Bogdanova, V. S., Tsibezov, V. V., Gibadulin, R. A., Musiyenko, M. I., Alekseyev, K. P., Grebennikova, T. V., Verkhovsky, O. A., Zabeezhnyi, A. D. and Aliper T. I. (2006) ‘Enzyme immunoassay for detection of porcine circovirus type 2, by using the recombinant capsid protein ORF‑2’ [Immunofermentnyy metod vyyavleniya antitel k tsirkovirusu sviney vtorogo tipa s primeneniem rekombinantnogo kapsidnogo belka ORF‑2]. Problems of Virology [Problemy virusologii], 51(5), pp. 44–48. [in Russian]

Stevenson, G. W., Kiupel, M., Mittal, S. K., Choi, J., Latimer, K. S. and Kanitz, C. L. (2001) ‘Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors’, Journal of Veterinary Diagnostic Investigation, 13(1), pp. 57–62. doi: