Issue 1

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 4, Issue 1, March 2018, Pages 15–17

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF THE INFLUENCE OF BACTERICIDAL PREPARATION ON CELL CULTURES

Kovalenko V. L. 1, Harkavenko V. M. 1, Kukhtyn M. D. 2, Ponomarenko G. V. 3, Ihnatieva T. M. 3

1 State Scientific Control Institute of Biotechnology and Strains of Microorganisms,
Kyiv, Ukraine, e-mail: kovalenkodoktor@gmail.com

2 Ivan Puluj Ternopil National Technical University, Ternopil, Ukraine; е-mail: kuchtyn@yandex.ua

3 Kharkiv State Zooveterinary Academy, Kharkiv, Ukraine, e-mail: gpkh1966@gmail.com

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Citation for print version: Kovalenko, V. L., Harkavenko, V. M., Kukhtyn, M. D., Ponomarenko, G. V. and Ihnatieva, T. M. (2018) ‘Study of the influence of bactericidal preparation on cell cultures’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 4(1), pp. 15–17.

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Citation for online version: Kovalenko, V. L., Harkavenko, V. M., Kukhtyn, M. D., Ponomarenko, G. V. and Ihnatieva, T. M. (2018) ‘Study of the influence of bactericidal preparation on cell cultures’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 4(1), pp. 15–17. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue1/oJVMBBS_2018041_015-014.pdf

Summary. Pressing problem today is the existence of a large number of microorganisms — causative agents of viral diseases, highly resistant to bactericidal preparations. At the same time, an important direction of research is the study of the toxic effects of new disinfectants not only on the organism of laboratory animals, but also on cell cultures, which is more humane and meets the modern standards of bioethics. That’s what determined the purpose of research — the study of the antiviral and toxic effects of the bactericidal preparation ‘Barez’ on the Aujeszky’s disease virus strain ‘Clone-B’, tested and adapted to the continuous cell cultures SNEV and PTP. When conducting research there were studied methods for assessing toxic effects of antibacterial drug ‘Barez’ on the cell cultures of swine origin SNEV, PTP, and its effect on the titer of infectious activity of the vaccine strain ‘Clone-B’ of Aujeszky’s disease virus from the collection of strains of the State Scientific Control Institute of Biotechnology and Strains of Microorganisms. When studying the antiviral properties of the bactericidal preparation on the Aujeszky’s disease virus ‘Clone-B’ strain, tested and adapted for continuous cell cultures SNEV and PTP, there was determined that 0.2–0.5% solutions of the drug ‘Barez’ are not toxic when effecting cell cultures, but exhibit inactivating effect on the Aujeszky’s disease virus. Therefore, it is possible to use it for preventive disinfection of objects of veterinary medicine in the presence of animals in recommended concentrations and exposures.

Keywords: bactericidal drug ‘Barez’, cell cultures, toxicity, Aujeszky’s disease virus, silver nanoparticles, benzalkonium chloride, essential oils

References:

Ababutain, I. M. (2011) ‘Antimicrobial activity of ethanolic extracts from some medicinal plant’, Australian Journal of Basic and Applied Sciences, 5(11), pp. 678–683. Available at: http://www.ajbasweb.com/old/ajbas/2011/November-2011/678-683.pdf

Deyneka, S. E. (1999) ‘Comparative express in vitro assessment of the degree of toxicity and danger of a number of metal salts’ [Sravnitel’naya ekspress-otsenka in vitro stepeni toksichnosti i opasnosti ryada soley metallov], Hygiene of Inhabited Places [Gigiena naselennykh mest], 35, pp. 549–555. [in Russian]

Kovalenko, V. L, Belokon’, V. I., Lopatko, K. G. and Ponomarenko, G. V. (2010) ‘Assessment microbicide toxicity on cell cultures origin pork SNEK, PTP’ [Otsinka toksychnosti bakterytsydnykh zasobiv na klitynnykh kulturakh svyniachoho pokhodzhennia SNEV, PTP], Problems of Zooengineering and Veterinary Medicine [Problemy zooinzhenerii ta veterynarnoi medytsyny], 22(2.1), pp. 114–118. [in Ukrainian]

Kovalenko, V. L. and Nedosiekov, V. V. (2011) Methodical approaches to the control of disinfectants for veterinary medicine [Metodychni pidkhody shchodo kontroliu dezinfikuiuchykh zasobiv dlia veterynarnoi medytsyny]. Kyiv. [in Ukrainian]

Pathmanathan, M. K., Uthayarasa, K., Jeyadevan, J. P. and Jeyaseelan, E. C. (2010) ‘In vitro antibacterial activity and phytochemical analysis of some selected medicinal plants’, International Journal of Pharmaceutical and Biological Archives, 1(3), pp. 291–299. Available at: http://ijpba.info/ijpba/index.php/ijpba/article/view/138

Stadnytska, N. Ye., Komarovska-Porokhniavets, O. Z., Kishchak, Kh. Ya., Mykoliv, O. B., Lytvyn, B. Ya., Konechna, R. T. and Novikov, V. P. (2011) ‘Plants with antimicrobial properties’ [Roslyny z protymikrobnymy vlastyvostiamy], Herald of the Lviv Polytechnic National University. Chemistry, Technology Substances and Their Use [Visnyk Natsionalnoho universytetu ‘Lvivska politekhnika. Khimiia, tekhnolohiia rechovyn ta yikh zastosuvannia], 700, pp. 111–116. Available at: http://ena.lp.edu.ua/bitstream/ntb/10547/1/28.pdf. [in Ukrainian]