Issue 4

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 4, Issue 4, December 2018, Pages 12–15

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

GENOTYPING OF FRANCISELLA TULARENSIS ISOLATES FROM UKRAINE

Zlenko O. B. 1, Durr A. 2, Schwarz J. 2, Vydaiko N. B. 3, Gerilovych A. P. 1

1 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: oksana.ceratium@gmail.com

2 Bundeswehr Institute of Microbiology, Munich, Germany

State Institution ‘Public Health Center of the Ministry of Health of Ukraine’, Kyiv, Ukraine

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Citation for print version: Zlenko, O. B., Durr, A., Schwarz, J., Vydaiko, N. B. and Gerilovych, A. P. (2018) ‘Genotyping of Francisella tularensis isolates from Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 4(4), pp. 12–15.

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Citation for online version: Zlenko, O. B., Durr, A., Schwarz, J., Vydaiko, N. B. and Gerilovych, A. P. (2018) ‘Genotyping of Francisella tularensis isolates from Ukraine’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 4(4), pp. 12–15. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue4/oJVMBBS_2018044_012-015.pdf

Summary. The aim of the work is to provide fingerprinting of 20 tularemia isolates collected at the territory of Ukraine and find their relationships with other typed tularemia isolates. The 20 randomly sampled Francisella tularensis thermolysates were collected during 1997–2016 on the territory of Ukraine. The panel included samples from 11 regions of Ukraine. Samples were collected from patients (3 samples), rodents (8 samples) and lagomorphs (1 sample), ticks (6 samples) and environment (2 samples). The genotyping and processing of data was carried out using the MLVA 12+1 method. Data were statistically processed using the method of unweighted pair group method with arithmetic mean-clustering (UPGMA). It was found that all specimens belong to the same subgenus F. tularensis subsp. holarctica and belong to three genotypes: Russian-Azerbaijan (5 samples), Czechoslovakia (1 sample) and European (14 samples) one. The European genotype is inherent in the 70% of studied samples almost in every studied year and can be named as the leading genotype that distributed on the territory of all Ukraine and is related to European genotypes of F. tularensis.

Keywords: MLVA, tularemia, Ukraine, UPGMA, Francisella tularensis subsp. holarctica

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