Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 6, Issue 4, December 2020, Pages 13–16

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)


Buchatskyi L. P.

Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, e-mail:

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Citation for print version: Buchatskyi, L. P. (2020) ‘Prolonged hypoxia induced melanotic pseudotumors in the larvae of blood-sucking mosquitoes’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 6(4), pp. 13–16.

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Citation for online version: Buchatskyi, L. P. (2020) ‘Prolonged hypoxia induced melanotic pseudotumors in the larvae of blood-sucking mosquitoes’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 6(3), pp. 13–16. DOI: 10.36016/JVMBBS-2020-6-4-3.

Summary. It was found that the presence of mosquito eggs in artificially created conditions of prolonged hypoxia causes the appearance of numerous melanotic pseudotumors in the larvae hatching from such eggs. In the cells of melanotic pseudotumors multilayer concentric membrane-like structures were found in the cytoplasm. In the immediate vicinity of such membranes, small spherical virus-like particles (VLP) with a diameter of about 30 nm were observed. The possible role of hypoxia in the development of melanotic pseudotumors of mosquito larvae is discussed

Keywords: electron microscopy, virus-like particles, Aedes, Culicidae, Diptera, Ukraine


Bjørgen, H., Wessel, Ø., Fjelldal, P. G., Hansen, T., Sveier, H., Sæbø, H. R., Enger, K. B., Monsen, E., Kvellestad, A., Rimstad, E. and Koppang, E. O. (2015) ‘Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar)’, Veterinary Research, 46(1), p. 89. doi:

Buchatskyi, L. P. (2020) Invertebrate Virology [Virusolohiia bezkhrebetnykh]. Кyiv: DIA. [in Ukrainian]

Buchatskyi, L. P. and Sheremet, V. P. (1978) ‘Induction of melanotic tumors in blood-sucking mosquitoes’ [Induktsiya melanoticheskikh opukholey u krovososushchikh komarov], Abstract Information About Completed Research Projects in the Universities of the Ukrainian SSR [Referativnaya informatsiya o zakonchennykh NIR v vuzakh USSR], 12, p. 32. [in Russian]

Burdette, W. J. and Yoon, J. S. (1967) ‘Mutations, chromosomal aberrations, and tumors in insects treated with oncogenic virus’, Science, 155(3760), pp. 340–341. doi:

Centanin, L., Dekanty, A., Romero, N., Irisarri, M., Gorr, T. A. and Wappner, P. (2008) ‘Cell autonomy of HIF effects in Drosophila: Tracheal cells sense hypoxia and induce terminal branch sprouting’, Developmental Cell, 14(4), pp. 547–558. doi:

Christensen, B. M., Li, J., Chen, C.-C. and Nappi, A. J. (2005) ‘Melanization immune responses in mosquito vectors’, Trends in Parasitology, 21(4), pp. 192–199. doi:

Gonzalez, C. (2013) ‘Drosophila melanogaster: A model and a tool to investigate malignancy and identify new therapeutics’, Nature Reviews Cancer, 13(3), pp. 172–183. doi:

Harshbarger, J. C. and Taylor, R. L. (1968) ‘Neoplasms of Insects’, Annual Review of Entomology, 13, pp. 159–190. doi:

Hubálek, Z., Rudolf, I. and Nowotny, N. (2014) ‘Chapter Five — Arboviruses pathogenic for domestic and wild animals’, in Maramorosch, K. and Murphy, F. A. (eds.) Advances in Virus Research. Oxford: Academic Press, pp. 201–275. doi:

Lundquist, T. A. (2016) Expression of HIF-1 Alpha and HIF-1 Beta in Insects Throughout Juvenile Development. MSc thesis. North Dakota State University. Available at:

Minchenko, O. H., Kharkova, A. P., Minchenko, D. O. and Karbovskyi, L. L. (2015) ‘Effect of hypoxia on the expression of genes that encode some IGFBP and CCN proteins in U87 glioma cells depends on IRE1 signaling’, Ukrainian Biochemical Journal, 87(6), pp. 52–63. doi:

Minchenko, O. H., Riabovol, O. O., Tsymbal, D. O., Minchenko, D. O. and Ratushna, O. O. (2016) ‘Effect of hypoxia on the expression of nuclear genes encoding mitochondrial proteins in U87 glioma cells’, Ukrainian Biochemical Journal, 88(3), pp. 54–65. doi:

Osinsky, S., Zavelevich, M. and Vaupel, P. (2009) ‘Tumor hypoxia and malignant progression’, Experimental Oncology, 31(2), pp. 80–86. Available at:

Pagès, N. and Cohnstaedt, L. W. (2018) ‘8. Mosquito-borne diseases in the livestock industry’, in Garros, C., Bouyer, J., Takken, W., and Smallegange, R. C. (eds.) Pests and Vector-Borne Diseases in the Livestock Industry. Ecology and Control of Vector-borne Diseases, 5. Wageningen, The Netherlands: Wageningen Academic Publishers, pp. 195–219. doi:

Rachidi, M., Lopes, C., Benichou, J.-C., Hellio, R. and Maisonhaute, C. (2005) ‘Virus-like particle formation in Drosophila melanogaster germ cells suggests a complex translational regulation of the retrotransposon cycle and new mechanisms inhibiting transposition’, Cytogenetic and Genome Research, 111(1), pp. 88–95. doi:

Reuter, M. (1975) ‘Viruslike particles in Gyratrix hermaphroditus (Turbellaria: Rhabdocoela)’, Journal of Invertebrate Pathology, 25(1), pp. 79–95. doi:

Semin, B. V. and Il’in, Yu. V. (1994) ‘Extracellular virus-like particles retrotransposon gypsy (Mdg4) as an infectivity factor’ [Vnutrikletochnye virusopodobnye chastitsy retrotranspozona gypsy (Mdg4) kak faktor infektsionnosti], Reports of the Academy of Sciences [Doklady Akademii Nauk], 339(6), pp. 838–841. PMID: [in Russian]

Solov’ev, V. D., Khesin, Ya. E. and Bykovskiy, A. F. (1979) Essays on Viral Cytopathology [Ocherki po virusnoy tsitopatologii]. Moscow: Meditsina. [in Russian]

Tascedda, F. and Ottaviani, E. (2014) ‘Tumors in invertebrates’, Invertebrate Survival Journal, 11(1), pp. 197–203. Available at:

Yoshioka, K., Honma, H., Zushi, M., Kondo, S., Togashi, S., Miyake, T. and Shiba, T. (1990) ‘Virus-like particle formation of Drosophila copia through autocatalytic processing’, The EMBO Journal, 9(2), pp. 535–541. doi:

Yu, T., Koppetsch, B. S., Pagliarani, S., Johnston, S., Silverstein, N. J., Luban, J., Chappell, K., Weng, Z. and Theurkauf, W. E. (2019) ‘The piRNA response to retroviral invasion of the koala genome’, Cell, 179(3), pp. 632–643. doi:

Yudin, N. S., Aitnazarov, R. B. and Ermolaev, V. I. (2011) ‘Porcine endogenous retroviruses: Is the risk of transmission in xenografting great?’ [Endogennye retrovirusy svin’i: naskol’ko velik risk infektsii pri ksenotransplantatsii?], Vavilov Journal of Genetics and Breeding [Vavilovskiy zhurnal genetiki i selektsii], 15(2), pp. 340–350. Available at: [in Russian]

Zhang, Q., Huang, R., Hu, H., Yu, L., Tang, Q., Tao, Y., Liu, Z., Li, J. and Wang, G. (2020) ‘Integrative analysis of hypoxia-associated signature in pan-cancer’, iScience, 23(9), p. 101460. doi: