Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
8, Issue 1–2, May 2022, Pages 17–22
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DEVELOPMENT OF
DIFFERENTIATION METHOD FOR BOVINE HERPESVIRUS
SEROTYPES (BHV-1, BHV-4, BHV-5) USING POLYMERASE CHAIN REACTION
Al
Jabari M.
State Biotechnological
University, Kharkiv, Ukraine, e-mail: aljabari.r.muneer@gmail.com
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PDF (print version)
Citation for print version: Al Jabari, M. (2022)
‘Development of differentiation method for bovine herpesvirus
serotypes (BHV-1, BHV-4, BHV-5) using polymerase chain reaction’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 8(1–2), pp. 17–22.
Download
PDF (online version)
Citation for online version: Al Jabari, M. (2022)
‘Development of differentiation method for bovine herpesvirus
serotypes (BHV-1, BHV-4, BHV-5) using polymerase chain reaction’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 8(1–2), pp. 17–22.
DOI: 10.36016/JVMBBS-2022-8-1-2-3.
Summary. Infectious pneumoenteritis
of cattle is etiologically caused by viruses of different families and species.
Bovine herpesvirus type 1 —
infectious rhinotracheitis virus — is the
main and the most dangerous pathogen transmitted by cattle semen. At the same
time, recently, according to European scientists’ data, in addition to
this pathogen, other herpesviruses have been
circulating in cattle groups, in particular, bovine herpesvirused
of types 4 and 5. Studies have been conducted using molecular-genetic and bioinformatic methods. Based on the
analysis of the genomes of bovine herpesvirus of
types 1 (IBR virus), 4 and 5 we developed
primers BoHV-1 F/R, which flanks the DNA
fragment of the IBR virus with the length of
204 bp, BoHV-4 F/R,
which flanks the DNA fragment of bovine herpesvirus
type 4 with the length of 615 bp, and BoHV-5 F/R for bovine herpesvirus
type 5 DNA amplification with the formation of specific fragments
158 bp in length. The tests demonstrated
that primers specific for bovine herpesvirus of types
1, 4 and 5 can be used in multiplex amplification format and hybridized only
with specific DNA matrices of bovine herpesviruses. A
standard operating procedure ‘Indication of DNA of infectious bovine rhinotracheitis virus and bovine herpesviruses
of types 4 and 5 by polymerase chain reaction’ has
been developed
Keywords: infectious bovine rhinotracheitis,
infectious pneumoenteritis, cattle, primers
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