Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
9, Issue 3, September 2023, Pages 11–17
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDY OF MORPHOLOGICAL, KARYOLOGICAL,
BIOLOGICAL CHARACTERISTICS OF VIRUS-PRODUCING CELL LINES FLK-BLV
(FLK‑POL, FLK‑71,
FLK 50/100, FLK‑SBBL)
DURING LONG-TERM STORAGE IN THE CRYOBANK OF THE
NATIONAL COLLECTION OF THE NSC ‘IECVM’
Stegniy M. Yu., Stegniy B. T.,
Hrytsyna N. V.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: marina.stegniy@gmail.com
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PDF (print version)
Citation for print version: Stegniy, M. Yu.,
Stegniy, B. T., Hrytsyna, N. V.
(2023) ‘Study of morphological, karyological,
biological characteristics of virus-producing cell lines FLK-BLV
(FLK‑POL, FLK‑71,
FLK 50/100, FLK‑SBBL)
during long-term storage in the cryobank of the
National collection of the NSC ‘IECVM’’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 9(3),
pp. 11–17.
Download
PDF (online version)
Citation for online version: Stegniy, M. Yu.,
Stegniy, B. T., Hrytsyna, N. V.
(2023) ‘Study of morphological, karyological,
biological characteristics of virus-producing cell lines FLK-BLV
(FLK‑POL, FLK‑71,
FLK 50/100, FLK‑SBBL)
during long-term storage in the cryobank of the
National collection of the NSC ‘IECVM’’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 9(3), pp. 11–17. DOI: 10.36016/JVMBBS-2023-9-3-3.
Summary. Studies have shown that, morphologically, the
virus-producing cell line (FLK 50/100) consists
of epithelial-like cells with a perinuclear space around the nucleus, while the
FLK‑71 line is represented
by fibroblast-like cells with large nuclei. The karyology of FLK‑71 cell lines after long-term storage was
characterized by an increase in the range of chromosome number fluctuations
from 40 to 64 from the data sheet of these cells before freezing (from 42 to
56). The limits of chromosome number fluctuation in FLK 50/100
decreased and ranged from 40 to 68. The antigen-producing biological activity
of the subline of the reinoculated FLK-BLV culture (FLK‑Pol)
was preserved from passage 5 and further up to 22 passages (passage
period) during the studied storage periods, namely from 55.5 months (FLK‑Pol) to 201 months of storage (FLK‑SBBL) and 206.5 months (FLK 50/100)
in the conditions of the NSC ‘IECVM’ cryobank. However,
for the strain FLK 50/100, up to
10 passages after thawing are required to fully recover
the titers of its biological activity
Keywords: virus antigens, mitotic activity,
immunodiffusion test
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