Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume 1, Issue 2, June 2015, Pages 32–33
ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)
DEVELOPMENT OF SOFTWARE FOR CALCULATING THE BIOLOGICAL ACTIVITY OF MYCOBACTERIAL ALLERGENS
Bilushko V. V., Zavgorodniy A. I.
Bilushko E. V.
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Citation for print version: Bilushko V. V., Zavgorodniy A. I. and Bilushko E. V. (2015) ‘Development of software for calculating the biological activity of mycobacterial allergens’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(2), pp. 32–33.
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Citation for online version: Bilushko V. V., Zavgorodniy A. I. and Bilushko E. V. (2015) ‘Development of software for calculating the biological activity of mycobacterial allergens’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(2), pp. 32–33. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue2/oJVMBBS_2015012_032-033.pdf
Summary. The paper contains the results of the development and production testing of the software tool calculation for biological activity of mycobacterial allergens for veterinary use. Purpose — to develop the software for calculating of the biological activity of mycobacterial allergens, and conduct it's testing of industrial development in the catch during the control of commercial series of tuberculin purified (PPD) for mammals (n = 28).
Software design system was used cba platform .NET Framework 4.5, the programming language C# 5.0, the development environment Microsoft Visual Studio 2013, as well as WPF technology. Clinical studies were performed under conditions of Sumy State Biofactory by the parallel calculation of tuberculin purified activity (PPD) for mammals in the standard solution (series number = 28) using the developed program cba and calculation method of biological activity according to the current TC U 24.4-00497087-107:2011 ‘Tuberculіn dry purified (PPD) for mammals (National standard)’.
As a result, the calculation of the biological activity of 28 experimental series of tuberculin for mammals according to the procedure specified in regulatory documents and with the help of the developed software cba demonstrated activity of the preparation at the level 53 746 U/cm3 in standard solution.
The computer program cba for the determination of the biological activity of mycobacterial allergens provides accurate and timely calculation of the amount of unit content in the volume 1.0 cm3 of the standard solution of purified tuberculin (PPD) for mammals.
Perspectives for future research will include conduction of the further testing of the software for allergen dry purified from atypical mycobacteria (AAM) and avian purified tuberculin (PPD) in the production environment, as well as in the development of targeted programs for different operating processes that require complex mathematical calculations in the manufacture process of different preparation dilutions, as the conditions of the producing and for research purpose.
Keywords: biological activity, software, units (U), mycobacterial allergens, tuberculin
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