Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
2, Issue 1, May 2016, Pages 16–19
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
REPRODUCTION CAPABILITY OF
BOVINE LEUCOSIS FIELD ISOLATES IN SOME CELL CULTURES
Gorbatenko
S. K., Stegniy M. Yu., Korneykov A. N.
National
Scientific Center «Institute of Experimental and Clinical Veterinary
Medicine», Kharkiv, Ukraine, e-mail: leylab@vet.kharkov.ua
Download
PDF (print version)
Citation for
print version: Gorbatenko, S.
K., Stegniy, M. Yu. and Korneykov
A. N. (2016) ‘Reproduction capability of bovine leucosis field isolates
in some cell cultures’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 2(1),
pp. 16–19.
Download
PDF (online version)
Citation for
online version: Gorbatenko, S. K.,
Stegniy, M. Yu. and Korneykov A. N. (2016) ‘Reproduction capability of
bovine leucosis field isolates in some cell cultures’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 2(1), pp. 16–19. Available at: http://jvmbbs.kharkov.ua/archive/2016/volume2/issue1/oJVMBBS_2016021_016-019.pdf
Summary. The
article observes capability of cow embryo lung (CEL), calf coronary vessel
(CVC), sheep kidney (SK), and calf kidney (CK) passaged cells cultures to
reproduce bovine leukemia virus after integration of pathogen field isolates.
The presence of pathogen proviral DNA was detected by
molecular-genetic studies at different cultivation stages. Increasing of
hemagglutinin titer in CVC, SK, CK, and CEL cell cultures was
established in between 5–6 passages.
Keywords: leucosis,
cattle, cells cultures, CVC, SK, CK, field isolates, reproduction capability
References:
Burba, L. G., Valikhov, A. F.,
Shishkov, V. P., Kiselev, N. V. and
Sorokina, A. A. (1996) ‘Preparations for bovine leukemia virus
diagnostics’ [Preparaty dlya diagnostiki leykoza krupnogo rogatogo
skota], Kursk biofactory. To the 100th anniversary of the biological
industry in Russia [Kurskaya biofabrika. K 100-letiyu biologicheskoy
promyshlennosti Rossii]. Kursk: GUIPP ‘Kursk’,
pp. 451–478. [in Russian]
Busol, V. O., Miahkykh, N. V.,
Zdanevych, P. P., Shapovalova, O. V. and
Tsymbal, V. I. (2000) ‘Production of trail series of
antileukemic vaccine and its examination in laboratory conditions’ [Vyhotovlennia
doslidnykh serii protyleikoznoi vaktsyny ta perevirka yii v laboratornykh
umovakh], Scientific Bulletin of the National Agrarian University [Naukovyi
visnyk Natsionalnoho ahrarnoho universytetu], 28, pp. 120–122.
[in Ukrainian]
DSSU [State Committee for Technical Regulation and Consumer Policy]
(2005) DSTU 4483:2005. Veterinary immunobiological preparations. Methods for
determination of bacterial and fungous contamination [Preparaty veterynarni
imunobiolohichni. Metody vyznachannia bakterialnoi i hrybkovoi kontaminatsii].
Kyiv: Derzhspozhyvstandart Ukrainy
Stegniy, B. T., Fisenko, S. A.,
Stegniy, M. Y, Dunaev, Y. K., Konovalov, A. V.
and Babanin, N. I. (2011) ‘Improvement of the technology of
antigen for serological diagnosis of bovine leukemia in immunodiffusion test
production’ [Udoskonalennia tekhnolohii vyrobnytstva antyhenu dlia
serolohichnoi diahnostyky leikozu velykoi rohatoi khudoby v reaktsii
imunodyfuzii], Veterinary Medicine [Veterynarna medytsyna], 95,
pp. 227–229. Available at: http://jvm.kharkov.ua/sbornik/95/5_99.pdf.
[in Ukrainian]
Syurin, V. N., Samuylenko, A. Y.,
Solov’ev, B. V. and Fomina, N. V. (1998) Viral
diseases of animals [Virusnye bolezni zhivotnykh]. Moscow: VNITIBP. [in
Russian]
Tsymbal, V. I.,
Kiprich, V. V., Busol, V. A., Shikov, V. P.,
Burba, L. G. and Valikhov, A. F. (1990) A method of
antigen derivation for bovine leukemia virus diagnostics in immunodiffusion
test [Sposob polucheniya antigena dlya diagnostiki leykoza krupnogo rogatogo
skota v reaktsii immunodiffuzii]. USSR Author’s Certificate no:
SU1585951. [in Russian]
