Issue 1

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 1, March 2015, Pages 32–38

ISSN XXXX-XXXX (print version) ISSN ISSN 2411-0388 (online version)

MODERN TURKISH APPROACHES TO SOILS' DECONTAMINATION FROM ANTHRAX' AGENT WITH ATTENTION TO UKRAINE NEEDS

Sahin M., Buyuk F., Celik E.

University of Kafkas, Faculty of Veterinary Medicine, Department of Microbiology, Kars, Turkey, e-mail: mitats@hotmail.com, fatihbyk08@hotmail.com

Buzun A., Koltchik H., Rahimov J.

National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine, e-mail: epibuz2@yahoo.co.in

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Citation for print version: Sahin, M., Buyuk, F., Celik, E., Buzun, A. I., Koltchik, H. V. and Rahimov, J. (2015) ‘Modern Turkish approaches to soils' decontamination from anthrax' agent with attention to Ukraine needs’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(1), pp. 32–38.

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Citation for online version: Sahin, M., Buyuk, F., Celik, E., Buzun, A. I., Koltchik, H. V. and Rahimov, J. (2015) ‘Modern Turkish approaches to soils' decontamination from anthrax' agent with attention to Ukraine needs’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(1), pp. 32–38. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue1/jvmbbso2015011_032-038.pdf

Summary. The Turkish experience on soil decontamination is based by modern conception of anthrax’ driving forces – about clue role of bacteriophages in natural history of anthrax agent. This approach (as Kafkas University SOP) was proposed to approbation by Turkish-Ukrainian scientifically cooperation in framework of EU-project for soil decontamination.

Study was performed by the bacteriological (bacteria and phages isolation, identification, and cultivation, PCR) and epidemiological (surveillance with sampling, epidemiology analysis) methods. Samples were collected using basic methods from animal burial sites in Eastern Parts of Turkey (Kars, n=5) and Ukraine (Gorlovka, n=1). Soil desporulation conducted by germination of spores and elimination of vegetative B. anthracis with phages or peracetic acid. All procedures were standardized by SOPs of Kafkas University as in Turkish experience for soil desporulation.

The SOPs for surveys of the soil anthracic spores and phages, and soil desporulation (7 sites of sampling, 27 specimens) is effective, ecological friendly and economically low-cost. On two trials in Turkey (burial area “Külveren”, S≈30m2) and Ukraine (sample of soil from burial area “Gorlovka-2”, weight 150 g) the contaminated soils (5×104 and 5×105, respectively) were de-sporulated during 24 hr. and hadn’t residual viable spore in germinator presence even. Epidemiological analysis proves the Donbas region as most dangerous by anthrax’ prognosis.

Turkish method is very useful to application in Ukraine, especially on the liberated territories of Donbas.

Keywords: spores of Bacillus anthracis, monitoring of soils contamination, decontamination procedure, Turkish technology, Ukrainian needs

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