Issue 4
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
1, Issue 4, December 2015, Pages 5–8
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
Studying of phylogenetic relationships of leukemia virus with other
retroviruses in cattle
Limanskaya O. Yu., Gema I.
A., Gorbatenko S. K., Gerilovych
A. P.
National
Scientific Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: antger2011@gmail.com
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PDF (print version)
Citation for print version: Limanskaya, O. Yu.,
Gema, I. A., Gorbatenko, S.
K. and Gerilovych, A. P. (2015) ‘Studying of phylogenetic relationships of leukemia virus with other
retroviruses in cattle’, Journal
for Veterinary Medicine, Biotechnology and Biosafety,
1(4), pp. 5–8.
Download
PDF (online version)
Citation for
online version: Limanskaya, O. Yu., Gema, I. A., Gorbatenko, S. K. and Gerilovych,
A. P. (2015) ‘Studying of phylogenetic
relationships of leukemia virus with other retroviruses in cattle’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(4),
pp. 5–8. Available at:
http://jvmbbs.kharkov.ua/archive/2015/volume1/issue4/oJVMBBS_2015014_005-008.pdf
Summary.
Bovine leukemia virus (BLV) is one of the
retroviruses, which genetically, structurally and functionally related to viruses of human
T-cell leukemia. BLV is a very convenient model for studying the
pathogenesis of human leukemia. Genomes of retroviruses have high variability
levels due to lack of a mechanism of correct errors that occur when copying
matrix during replication, and possible genetic recombination. In this regard,
the study of the genetic variability of the virus is one of the major
objectively for biological monitoring. At this time, molecular genetic analysis
(polymerase chain reaction (PCR)) is a necessary part
of phylogenetic research. The aim of this work was to
study the variability of the bovine leukemia virus, to establish phylogenetic relationships between isolates sequenced
bovine leukemia virus, which circulates in farms of different regions in
Ukraine, with other animals retroviruses. The sampling of clinical material
from cattle farms was conducted in different geographical regions in Ukraine
and extracted proviral BLV
DNA. Totally 831 samples of peripheral blood were collected and tested from
cattle farms in Kharkiv region, 10 samples — Kirovohrad region; 10 samples — Donetsk region; 41
samples — Crimea, Simferopol region; 10 — samples of Poltava
region. Sequenced fragments of env gene of bovine
leukemia virus proviral DNA, circulating in different
geographical regions in Ukraine were analyzed. Established isolates of bovine
leukemia virus, circulating in Ukraine, belonging to the Euro-Asian subtype.
Proved genetic affinity of leukemia virus and bovine syncytial
virus, Jembrana disease virus and bovine
immunodeficiency virus.
Keywords: DNA, phylogeographic relationships, polymerase chain reaction,
sequencing, retroviruses, virus bovine leukemia
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