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Issue 4

Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 1, Issue 4, December 2015, Pages 16–21

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

Phylogenetic and molecular genetic studies of porcine circovirus type II

Rudova N. G., Smolyaninova Ye. V., Lymanska O. Yu., Zlenko O. B.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: antger@vet.kharkov.ua

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Citation for print version: Rudova, N. G., Smolyaninova, Ye. V., Lymanska, O. Yu. and Zlenko, O. B. (2015) ‘Phylogenetic and molecular genetic studies of porcine circovirus type II’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 1(4), pp. 16–21.

Download PDF (online version)

Citation for online version: Rudova, N. G., Smolyaninova, Ye. V., Lymanska, O. Yu. and Zlenko, O. B. (2015) ‘Phylogenetic and molecular genetic studies of porcine circovirus type II’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 1(4), pp. 16–21. Available at: http://jvmbbs.kharkov.ua/archive/2015/volume1/issue4/oJVMBBS_2015014_016-021.pdf

Summary. The multiple farms all over the world are affected with porcine circovirus (PCV). This disease has been occurred in Ukraine. This work has been aimed to perform the phylogenetic and molecular genetic studies of porcine circovirus type II that circulate in Ukraine. This study involves wide spectrum of the molecular techniques, including PCR, RT‑PCR and sequencing of variable loci with the next following phylogenetic analysis. The phylogenetic relationships of porcine circovirus type II that circulate in different geographical regions were studied. The prospect of phylogenetic analysis for genotyping and molecular marking of microorganisms was demonstrated. The porcine circovirus type II indication by the real-time PCR method was described.

Keywords: phylogenetic analysis, real-time PCR, porcine circovirus type II

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