Issue 3
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 3, September 2017, Pages 5–9
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDYING OF PHYLOGENETIC
RELATIONSHIPS OF LEUKEMIA VIRUS WITH OTHER RETROVIRUSES IN CATTLE
Gerilovych A. P., Limanska O. Yu.,
Kulyk I. A., Gorbatenko S. K.
National Scientific Center ‘Institute of Experimental
and Clinical Veterinary Medicine’, Kharkiv,
Ukraine, e-mail: antger2011@gmail.com
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PDF (print version)
Citation for print version: Gerilovych, A. P., Limanska, O. Yu., Kulyk, I. A.
and Gorbatenko, S. K. (2017) ‘Studying of
phylogenetic relationships of Leukemia virus with other retroviruses in cattle’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 3(3), pp. 5–9.
Download
PDF (online version)
Citation for online version: Gerilovych, A. P., Limanska, O. Yu., Kulyk, I. A.
and Gorbatenko, S. K. (2017) ‘Studying of
phylogenetic relationships of Leukemia virus with other retroviruses in cattle’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety. [Online] 3(3), pp. 5–9. Available at:
http://jvmbbs.kharkov.ua/archive/2017/volume3/issue3/oJVMBBS_2017033_005-009.pdf
Summary. Bovine leukemia virus (BLV)
is one of the retroviruses, which genetically, structurally and functionally
related with viruses of human T‑cell leukemia. BLV
is very convenient model for studying the pathogenesis of humans
leukemia. Genomes of retroviruses is high variability due to lack of a
mechanism of correct errors that occur when copying matrix during replication,
and possible genetic recombination. In this regard, the study of the genetic
variability of the virus is one of the major objective for biological monitoring. At this time
molecular genetic analysis (polymerase chain reaction
(PCR) is a necessary part of phylogenetic research.
The aim of this work was to study the variability of the bovine leukemia virus,
establishing phylogenetic relationships between isolates sequenced bovine
leukemia virus, which circulates in farms of different regions in Ukraine, with
other animals retroviruses. Was conducted the sampling of clinical material
from cattle farms from different geographical regions in Ukraine and extracted proviral BLV DNA. Total received
831 samples of peripheral blood from cattle farms in Kharkiv
region, 10 samples — Kirovohrad
region; 10 samples — Donetsk region; 41 sample —
Crimea, Simferopol region; 10 samples — Poltava region.
Sequenced gene fragments of env sequences of bovine leukemia virus proviral
DNA, circulating in different geographical regions in Ukraine. Established
isolates of bovine leukemia virus, circulating in Ukraine, belonging to the
Euro-Asian subtype. Proved genetic affinity of leukemia virus and bovine
syncytial virus, Jembrana disease virus and bovine
immunodeficiency virus.
Keywords: DNA, phylogeographic relationships, polymerase chain reaction, sequencing, retroviruses, virus bovine leukemia
References:
Caraguel, C.,
Stryhn, H., Gagné, N.,
Dohoo, I. and Hammell, L.
(2009) ‘Traditional descriptive analysis and novel visual representation
of diagnostic repeatability and reproducibility: Application to an infectious
salmon anaemia virus RT-PCR
assay’, Preventive Veterinary Medicine, 92(1–2),
pp. 9–19. http://dx.doi.org/10.1016/j.prevetmed.2009.07.011
Chang, Z. R., Jin, M., Liu, N., Xie, H. P.,
Cui, S. X., Zhang, Q., Duan, Z. J.
(2009) ‘Analysis of epidemiologic feature and genetic sequence of Sapovirus in China’, Chinese Journal of Virology [Bing Du Xue Bao], 25(2), pp. 113–116. PMID: 19678565. [in Chinese]
Darlix, J.
and Spahr, P. (1983) ‘High spontaneous
mutation rate of Rons sarcoma virus demonstrated by
direct sequencing of the RNA genome’, Nucleic Acids Research,
11(17), pp. 5953–5967. http://dx.doi.org/10.1093/nar/11.17.5953
Dube, S., Bachman, S., Poiesz, B., Ferrer, J., Esteban, E.,
Choi, D., Love, J. and Spicer, T. (1997) ‘Degenerate and
specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma
virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from
cattle and primates from around the world’, Journal of General
Virology, 78(6), pp. 1389–1398. http://dx.doi.org/10.1099/0022-1317-78-6-1389
Giammarioli, M.,
Pellegrini, C., Casciari, C.,
Rossi, E. and De Mario, G. (2008) ‘Genetic diversity of
bovine viral diarrhea virus 1: Italian isolates clustered in at least
seven subgenotypes’, Journal of Veterinary
Diagnostic Investigation, 20(6), pp. 783–788. http://dx.doi.org/10.1177/104063870802000611
Katz, R. and Skalka, A. (1990)
‘Generation of diversity in retroviruses’, Annual Review of
Genetics, 24(1), pp. 409–443. http://dx.doi.org/10.1146/annurev.ge.24.120190.002205
Licursi, M.,
Inoshima, Y., Wu, D., Yokoyama, T.,
González, E. and Sentsui, H. (2003)
‘Provirus variants of bovine leukemia virus in naturally infected cattle
from Argentina and Japan’, Veterinary Microbiology, 96(1),
pp. 17–23. http://dx.doi.org/10.1016/s0378-1135(03)00202-5
Manini, P.,
De Palma, G. and Mutti, A. (2007)
‘Exposure assessment at the workplace: Implications of biological
variability’, Toxicology Letters, 168(3), pp. 210–218. http://dx.doi.org/10.1016/j.toxlet.2006.09.014
Meyerhans, A.,
Cheynier, R., Albert, J., Seth, M.,
Kwok, S., Sninsky, J., Morfeldt-Månson, L.,
Asjö, B. and Wain-Hobson, S.
(1989) ‘Temporal fluctuations in HIV quasispecies
in vivo are not reflected by sequential HIV isolations’, Cell,
58(5), pp. 901–910. http://dx.doi.org/10.1016/0092-8674(89)90942-2
Milos, P. (2009) ‘Emergence of single-molecule sequencing and
potential for molecular diagnostic applications’, Expert Review of
Molecular Diagnostics, 9(7), pp. 659–666. http://dx.doi.org/10.1586/erm.09.50
Parvin, J. D.,
Moscona, A., Pan, W. T., Leider, J. M. and Palese, P.
(1986) ‘Measurement of the mutation rates of animal viruses:
influenza A virus and poliovirus type 1’, Journal of
Virology, 59(2),
pp. 377–383. Available at: http://jvi.asm.org/content/59/2/377.full.pdf
Steinhauer, D.
and Holland, J. (1987) ‘Rapid evolution of RNA viruses’, Annual
Review of Microbiology, 41(1), pp. 409–431. http://dx.doi.org/10.1146/annurev.mi.41.100187.002205
Steinhauer, D. A.,
de la Torre, J. C., Meier, E. and Holland, J. J.
(1989) ‘Extreme heterogeneity in populations of vesicular stomatitis
virus’, Journal of Virology, 63(5), pp. 2072–2080. Available at: http://jvi.asm.org/content/63/5/2072.full.pdf
Wendel, J. F.
and Doyle, J. J. (1998) ‘Phylogenetic incongruence: window into
genome history and molecular evolution’, in: Soltis, D. E.,
Soltis, P. S., Doyle, J. J.
(eds.) Molecular Systematics of
Plants II. Boston: Springer, pp. 265–296. http://dx.doi.org/10.1007/978-1-4615-5419-6_10
Willems, L.,
Thienpont, E., Kerkhofs, P.,
Burny, A., Mammerickx, M.
and Kettmann, R. (1993) ‘Bovine leukemia
virus, an animal model for the study of intrastrain variability’,
Journal of Virology, 67(2),
pp. 1086–1089. Available at: http://jvi.asm.org/content/67/2/1086.full.pdf
