Issue 4
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 4, December 2017, Pages 13–15
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
RESEARCH OF THE INFLUENCE
OF DISINFECTANTS ON THE RATE
OF ABSORPTION OF OXYGEN BY
CELLS OF ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS BACTERIA
Ponomarenko G. V. 1,
Kovalenko V. L. 2, Ponomarenko O. V. 1, Balackiy
Yu. O. 3
1 Kharkiv State Zooveterinary Academy, Kharkiv,
Ukraine, e-mail: gpkh1966@gmail.com
2 State Scientific Control Institute of Biotechnology
and Strains of Microorganisms, Kyiv, Ukraine, e-mail: kovalenkodoktor@gmail.com
3 Bila Tserkva
National Agrarian University, Bila Tserkva Ukraine
Download
PDF (print version)
Citation for print version: Ponomarenko, G. V.,
Kovalenko, V. L., Ponomarenko,
O. V. and Balackiy, Yu. O. (2017) ‘Research of the influence of
disinfectants on the rate of
absorption of oxygen by cells
of Escherichia coli and Staphylococcus aureus bacteria’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 3(4), pp. 13–15.
Download
PDF (online version)
Citation for online version: Ponomarenko, G. V.,
Kovalenko, V. L., Ponomarenko,
O. V. and Balackiy, Yu. O. (2017) ‘Research of the influence of
disinfectants on the rate of
absorption of oxygen by cells
of Escherichia coli and Staphylococcus aureus bacteria’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 3(4), pp. 13–15. Available at:
http://jvmbbs.kharkov.ua/archive/2017/volume3/issue4/oJVMBBS_2017034_013-015.pdf
Summary. The development and implementation of modern complex
disinfectants involves the study of
their direct effect on the
test cultures of microorganisms, most often using
E. coli
and S. aureus. It is taken into
account several indicators of the
viability and resistance of bacterial
cells, including the rate oxygen
absorption. This is what led
to the purpose
of research — the study of
the effect of disinfectants on the rate
of oxygen absorption by the
test cultures of microorganisms E. coli and S. aureus. In the experiments, the effect of
disinfectants ‘Orgasept’,
‘Biochlor’, ‘Asept’,
and ‘Biolong’ on the test
cultures E. coli strain 1257, S. aureus
strain 209‑P from the strains collection
of the State
Scientific Control Institute of Biotechnology
and Strains of Microorganisms has been studied.
The rate of oxygen absorption
by bacteria cells E. coli and S. aureus under
the influence of disinfectants on them was
determined using the polarographic method (Frank et al., 1973). It has been established
that solutions of preparations 0.1% ‘Orgasept’, 0.1% ‘Biochlor’,
and 0.5% ‘Asept’
are effective disinfectants for E. coli and S. aureus bacteria. The use of
the ‘Biolong’ drug is less
effective than the use of
disinfecting drugs ‘Orgasept’, ‘Biochlor’,
and ‘Aseptic’. The sensitivity and resistance of microorganisms to disinfectants have been determined
and the optimal
effective concentrations and exposures of
these preparations have been investigated
for their further application in production conditions.
Keywords: test cultures of microorganisms, Escherichia coli, Staphylococcus aureus, resistance and viability, cell respiration
References:
Frank, G. M. (ed.) (1973) A
guide to the study of biological oxidation by the polarographic
method [Rukovodstvo po izucheniyu biologicheskogo okisleniya polyarograficheskim metodom]. Moscow: Nauka. [in Russian]
Kotsiumbas, I. Ya., Serhiienko, O. I.,
Kovalchyk, L. M., Khomiak, R. V.,
Kopiichuk, H. T. and Starchevskyi, M. K.
(2010) ‘Modern means of veterinary disinfection’ [Suchasni zasoby veterynarnoi dezinfektsii], Veterinary Medicine of Ukraine [Veterynarna medytsyna Ukrainy], 1, pp. 36–38. [in Ukrainian]
Kovalenko V. L. (2008) ‘Disinfectant
preparations use actual problems’ [Aktualni problemy zastosuvannia dezinfikuiuchykh preparativ], Veterinary
Biotechnology [Veterynarna biotekhnolohiia],
12, pp. 78–90. [in Ukrainian]
Kovalenko V. L. and Nedosiekov V. V.
(2011) Methodical approaches to the
control of disinfectants for veterinary medicine [Metodychni
pidkhody kontroliu dezinfikuiuchykh zasobiv dlia veterynarnoi medytsyny]. Kyiv. [in
Ukrainian]
Lakin, G. F.
(1990) Biometry [Biometriya]. 4th ed. Moscow: Vysshaya
shkola. ISBN 5060004716. [in
Russian]
Rabotnova, I. L.
and Pozmogova, I. N. (1979) Hemostatic cultivation and inhibition of
growth of microorganisms [Khemostatnoe kul’tivirovanie i ingibirovanie rosta mikroorganizmov]. Moscow: Nauka.
[in Russian]
Schnitzer, R.
and Grunberg, E. (1960) Drug resistance of microorganisms [Ustoychivost’
mikroorganizmov k lekarstvennym
veshchestvam]. Moscow: Inostrannaya
literatura. [in Russian]
