Issue 2
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
4, Issue 2, June 2018, Pages 44–45
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
CAENORHABDITIS ELEGANS PREDATION ON BACILLUS ANTHRACIS:
DECONTAMINATION OF SPORE CONTAMINATED SOIL WITH GERMINANTS
AND NEMATODES
Schelkle B. 1,
Choi Y. 2, Baillie L. W. 1,
Richter W. 2, Buyuk F. 3,
Celik E. 3, Wendling M. 2,
Sahin M. 3,
Gallagher T. 4
1 School of Pharmacy and Pharmaceutical
Sciences, Cardiff University, Cardiff, UK
2 Battelle Biomedical Research Center, Columbus,
OH, USA
3 Faculty of Veterinary Medicine, Department of Microbiology,
University of Kafkas, Kars, Turkey, e-mail: mitats@hotmail.com
4 Avila Scientific, Christiansburg, VA, USA
Download
PDF (print version)
Citation for print version: Schelkle, B.,
Choi, Y., Baillie, L. W., Richter, W., Buyuk, F.,
Celik, E., Wendling, M.,
Sahin, M. and Gallagher, T. (2018)
‘Caenorhabditis elegans
predation on Bacillus anthracis:
decontamination of spore contaminated soil with germinants
and nematodes’, Journal
for Veterinary Medicine, Biotechnology and Biosafety, 4(2),
pp. 44–45.
Download
PDF (online version)
Citation for online version: Schelkle, B.,
Choi, Y., Baillie, L. W., Richter, W., Buyuk, F.,
Celik, E., Wendling, M.,
Sahin, M. and Gallagher, T. (2018) ‘Caenorhabditis elegans
predation on Bacillus anthracis:
decontamination of spore contaminated soil with germinants
and nematodes’, Journal
for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 4(2), pp. 44–45. Available at: http://jvmbbs.kharkov.ua/archive/2018/volume4/issue2/oJVMBBS_2018042_044-045.pdf
Remediation
of Bacillus anthracis-contaminated
soil is challenging and approaches to reduce overall spore levels in
environmentally contaminated soil or after intentional release of the
infectious disease agent in a safe, low-cost manner are
needed.
B. anthracis spores are highly resistant to biocides, but once
germinated them become susceptible to traditional biocides or potentially even
natural predators such as nematodes in the soil environment. Here, we describe
a two-step approach to reducing B. anthracis spore load in soil during laboratory trials,
whereby germinants and Caenorhabditis elegans
nematodes are applied concurrently.
While
the application of germinants reduced B. anthracis
spore load by up to four logs depending on soil type, the addition of nematodes
achieved a further log reduction in spore count. These laboratory
based results suggest that the combined use of nematodes and germinants could represent a promising approach for the remediation
of B. anthracis
spore contaminated soil.
Keywords: anthrax, remediation, environmentally
friendly, Caenorhabditis elegans
N2, L-alanine, inosine
