Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
8, Issue 3–4, December 2022, Pages 8–12
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
FACTORS OF NON-SPECIFIC RESISTANCE OF BEE
HEMOLYMPH WHEN FEEDING PROBIOTIC DRUG ‘BILAKT’
Yevtushenko О. S.,
Desiatnykova О. V.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: elenasirenko88@gmail.com
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PDF (print version)
Citation for print version: Yevtushenko, О. S., Desiatnykova, О. V. (2022) ‘Factors
of non-specific resistance of bee hemolymph when feeding probiotic drug ‘Bilakt’’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 8(3–4),
pp. 8–12.
Download
PDF (online version)
Citation for online version: Yevtushenko, О. S., Desiatnykova, О. V. (2022) ‘Factors
of non-specific resistance of bee hemolymph when feeding probiotic drug ‘Bilakt’’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 8(3–4), pp. 8–12. DOI: 10.36016/JVMBBS-2022-8-3-4-2.
Summary. The paper presents the results of the feeding the
‘Bilakt’ probiotic on factors of
non-specific resistance in bees. Hemolymph samples were taken
on the 7th, 14th, and 21st days, and the
activity of lysozyme and phagocytosis, bactericidal activity were determined.
According to the research results, the lysozyme activity in the hemolymph of
3–5-day-old larvae and nurse bees of the experimental groups after
21 days was reliably 1.5 times higher than the control group and
before feeding. The increase in the hemolymph bactericidal factor in
3–5-day-old larvae, and in nurse bees — threefold on the 21st day
after the end of feeding with ‘Bilakt’
drug. Phagocytic activity before the beginning of the experiment in the
hemolymph of 3–5-day-old larvae was 39.5%, on the 1st day after the end
of feeding with ‘Bilakt’ it increased and
exceeded this indicator by 29%. The phagocytic index before feeding was
2.04 ± 0.11. On the 1st day after the end of
feeding, it increased by 18.4%, respectively. The phagocytic activity of
hemolymph of nurse bees was 45.8%. Accordingly, the phagocytic index was
2.2 ± 0.12. On the 1st day after the end of
‘Bilakt’ feeding, phagocytic activity
increased by 40.9%, the phagocytic index was 3.24 ± 0.1, which
was 32.1% higher than the initial level. Research results indicate that the use
of ‘Bilakt’ helps to improve the general
physiological condition of sick bees by stimulating the cellular and humoral
mechanisms of protection of insects from pathogens, i.e. increasing the
non-specific protective properties of both the body of 3–5-day-old larvae
and bee adults
Keywords: prevention, bactericidal activity, phagocytic
activity
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