Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
11, Issue 2, June 2025, Pages 24–31
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDY
OF BIOLOGICAL PROPERTIES OF SOME SPECIES OF ATYPICAL MYCOBACTERIA IN GUINEA
PIGS
Zavgorodniy A. I., Pozmogova S. A.,
Bilushko V. V., Sviridova K. O.,
Paliy A. P.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary
Medicine’, Kharkiv, Ukraine, e-mail: svetlanapozmogova@gmail.com
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PDF (print version)
Citation for print version: Zavgorodniy, A. I.,
Pozmogova, S. A., Bilushko, V. V.,
Sviridova, K. O. and Paliy, A. P.
(2025) ‘Study of biological
properties of some species of atypical mycobacteria in guinea pigs’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 11(2), pp. 24–31.
Download
PDF (online version)
Citation for online version: Zavgorodniy, A. I.,
Pozmogova, S. A., Bilushko, V. V.,
Sviridova, K. O. and Paliy, A. P.
(2025) ‘Study of biological
properties of some species of atypical mycobacteria in guinea pigs’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 11(2), pp. 24–31. DOI: 10.36016/JVMBBS-2025-11-2-5.
Summary. As the eradication of tuberculosis in farm animals progresses, the importance of atypical mycobacteria
(AM) and various types of mycobacteriosis is becoming
more significant. These mycobacteria can sensitize animals to tuberculin and,
in some cases, cause tuberculosis-like lesions, complicating the implementation
of anti-tuberculosis measures. The study aimed to assess the persistence of M. scrofulaceum, M. avium,
and M. phlei in guinea pigs after single
and three oral administrations, in comparison to M. bovis.
It also examined their ability to cause sensitization to allergens and the
duration of this effect based on bacterial load and elimination rates. Results
indicated that the persistence of M. avium,
M. scrofulaceum, and M. phlei in guinea pigs was temporary following oral
administration. These bacteria caused sensitization but did not lead to the
development of an infectious pathological process. After three administrations,
compared to a single administration, the excretion time of M. avium and M. scrofulaceum
in feces increased from 15 days to 30 days (M. phlei remained 15 days). Additionally, the
allergic response to the allergens from atypical mycobacteria extended from
60 days to 90 days (for M. phlei,
it increased from 30 days to 60 days). The persistence of M. bovis was a permanent colonization, the excretion of
the pathogen in the feces occurred after the dissemination of the pathological
process, i. e., in the later stages of the disease, the allergic state
persisted for up to 90 days. The duration of the allergic state,
persistence, and elimination depended on the bacterial load and the type of
mycobacteria
Keywords: persistence,
sensitizing, allergic state, pathogenicity
References:
Agdestein, A., Johansen, T. B., Kolbjørnsen, Ø., Jørgensen, A.,
Djønne, B. and Olsen, I. (2012)
‘A comparative study of Mycobacterium avium
subsp. avium and Mycobacterium avium subsp. hominissuis
in experimentally infected pigs’, BMC Veterinary Research, 8(1),
p. 11. doi: 10.1186/1746-6148-8-11.
CE (The Council of Europe). (1986)
European Convention for the Protection of Vertebrate Animals Used for
Experimental and Other Scientific Purposes. (European
Treaty Series, No. 123). Strasbourg: The Council
of Europe. Available at: https://conventions.coe.int/treaty/en/treaties/html/123.htm.
CEC (The Council of
the European Communities) (2010) ‘Directive 2010/63/EU of the European
Parliament and of the Council of 22 September 2010 on the protection of
animals used for scientific purposes’, The Official Journal of the
European Communities, L 276, pp. 33–79. Available
at: http://data.europa.eu/eli/dir/2010/63/oj.
Falkinham, J. O. (2021)
‘Ecology of nontuberculous mycobacteria’,
Microorganisms, 9(11), p. 2262. doi: 10.3390/microorganisms9112262.
Field, S. K. and Cowie, R. L.
(2006) ‘Lung disease due to the more common nontuberculous
mycobacteria’, Chest, 129(6), pp. 1653–1672. doi: 10.1378/chest.129.6.1653.
Goldstein, N.,
St. Clair, J. B., Kasperbauer, S. H.,
Daley, C. L. and Lindeque, B. (2019)
‘Nontuberculous mycobacterial musculoskeletal
infection cases from a tertiary referral center, Colorado, USA’, Emerging
Infectious Diseases, 25(6), pp. 1075–1083. doi: 10.3201/eid2406.181041.
Gomez-Buendia, A., Ortega, J., Diez-Guerrier, A.,
Rendahl, A., Saez, J. L.,
Bezos, J., Romero, B. and Alvarez, J. (2024) ‘Evaluating
the ability of non-tuberculous mycobacteria to induce
non-specific reactions in Bovine tuberculosis diagnostic tests in guinea pigs
and cattle’, Veterinary Microbiology, 298, p. 110250. doi: 10.1016/j.vetmic.2024.110250.
Griffith, D. E.
(2007) ‘Therapy of Nontuberculous mycobacterial
disease’, Current Opinion in Infectious Diseases, 20(2),
pp. 198–203. doi:
10.1097/QCO.0b013e328055d9a2.
Griffith, D. E., Aksamit, T.,
Brown-Elliott, B. A., Catanzaro, A., Daley, C., Gordin, F., Holland, S. M., Horsburgh, R., Huitt, G.,
Iademarco, M. F., Iseman, M.,
Olivier, K., Ruoss, S., Von Reyn, C. F., Wallace, R. J. and
Winthrop, K. (2007) ‘An official ATS/IDSA statement: Diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases’, American
Journal of Respiratory and Critical Care Medicine, 175(4),
pp. 367–416. doi: 10.1164/rccm.200604-571ST.
Harris, N. B. and Barletta, R. G. (2001) ‘Mycobacterium
avium subsp. paratuberculosis
in veterinary medicine’, Clinical Microbiology Reviews, 14(3),
pp. 489–512. doi: 10.1128/CMR.14.3.489-512.2001.
Heifets, L. (2004) ‘Mycobacterial infections caused by nontuberculous mycobacteria’, Seminars in
Respiratory and Critical Care Medicine, 25(3), pp. 283–295. doi: 10.1055/s-2004-829501.
Hendrikx, L., Van Hees, C. L. M., De Steenwinkel, J. E. M.,
Bax, H. I., Sprong, T.,
Mulder, B., Jansz, A., Van Griethuysen, A., Bosboom, R.,
Stemerding, A., Koetsier, M.,
Van Coevorden, M., Mourik, B. C.,
Quint, K. D., Ott, A., Van Soolingen, D., Kuipers, S.,
Van Crevel, R. and Van Ingen, J. (2022) ‘Treatment and outcome of
culture-confirmed Mycobacterium marinum
disease’, Open Forum Infectious Diseases, 9(4), p. ofac077. doi:
10.1093/ofid/ofac077.
Hewes, C. A.,
Schneider, R. K., Baszler, T. V.
and Oaks, J. L. (2005) ‘Septic arthritis and granulomatous synovitis caused by infection with Mycobacterium avium complex in a horse’, Journal of the
American Veterinary Medical Association, 226(12), pp. 2035–2038. doi: 10.2460/javma.2005.226.2035.
Jenkins, A. O.,
Gormley, E., Gcebe, N.,
Fosgate, G. T., Conan, A., Aagaard, C., Michel, A. L. and Rutten, V. P. M. G. (2018) ‘Cross
reactive immune responses in cattle arising from exposure to Mycobacterium bovis and non-tuberculous
mycobacteria’, Preventive Veterinary Medicine, 152,
pp. 16–22. doi: 10.1016/j.prevetmed.2018.02.003.
Kim, M.-J.,
Kim, K.-M., Shin, J.-I., Ha, J.-H., Lee, D.-H.,
Choi, J.-G., Park, J.-S., Byun, J.-H.,
Yoo, J.-W., Eum, S.,
Jung, M., Baik, S. C.,
Lee, W. K., Kang, H. L. and Shin, M.-K. (2021)
‘Identification of nontuberculous mycobacteria
in patients with pulmonary diseases in Gyeongnam,
Korea, using multiplex PCR and multigene
sequence-based analysis’, Canadian Journal of Infectious Diseases and
Medical Microbiology, 2021, p. 8844306. doi: 10.1155/2021/8844306.
Li, L., Maboni, G., Lack, A. and Gomez, D. E.
(2023) ‘Nontuberculous mycobacteria in horses:
A narrative review’, Veterinary Sciences, 10(7), p. 442. doi: 10.3390/vetsci10070442.
Lobo, Y. and Lun, K. (2021) ‘Tattoo-associated cutaneous Mycobacterium
mageritense infection: A case report and brief
review of the literature’, Case Reports in Dermatology, 13(3),
pp. 513–520. doi:
10.1159/000520255.
Manning, E. J. B.
and Collins, M. T. (2001) ‘Mycobacterium avium
subsp. paratuberculosis: pathogen,
pathogenesis and diagnosis’, Revue Scientifique et Technique de l’OIE,
20(1), pp. 133–150. doi:
10.20506/rst.20.1.1275.
MDVMSVIMAPFU (Main Department of Veterinary Medicine with the State Veterinary
Inspection of the Ministry of Agriculture and Food of Ukraine). (1994) Guidelines for the Diagnosis of Animal and Poultry
Tuberculosis [Nastanova po diahnostytsi tuberkulozu tvaryn ta ptytsi]. Kyiv. [in Ukrainian].
Mönki, J. A. K., Hewetson, M.,
Hahn, S., Vainio, K. and Skrzypczak, T. (2016) ‘Disseminated alimentary mycobacteriosis in the horse: A retrospective study of nine
cases’, Equine Veterinary Education, 28(11),
pp. 614–622. doi:
10.1111/eve.12393.
Mourad, A., Baker, A. W. and Stout, J. E. (2021)
‘Reduction in expected survival associated with Nontuberculous
mycobacterial pulmonary disease’, Clinical Infectious Diseases,
72(10), pp. e552–e557.
doi: 10.1093/cid/ciaa1267.
Park, D.-I., Kang, S. and Choi, S. (2021)
‘Evaluating the prevalence and incidence of bronchiectasis and nontuberculous mycobacteria in South Korea using the
nationwide population data’, International Journal of Environmental
Research and Public Health, 18(17), p. 9029. doi: 10.3390/ijerph18179029.
Pavlik, I., Ulmann, V. and Falkinham, J. O. (2022) ‘Nontuberculous mycobacteria: Ecology and impact on animal
and human health’, Microorganisms, 10(8), p. 1516. doi: 10.3390/microorganisms10081516.
Pavlik, I., Ulmann, V., Hubelova, D. and Weston, R. T. (2022)
‘Nontuberculous mycobacteria as Sapronoses: A review’, Microorganisms, 10(7),
p. 1345. doi: 10.3390/microorganisms10071345.
Silva, F. S.,
Lorenzett, M. P., Bianchi, M. V.,
Bastos, H. B. A., Larentis, G. R.,
Paul, L. G., Snel, G. G. M.,
Oliveira-Filho, J. P., Mattos, R. C.
and Sonne, L. (2019) ‘Mycobacterium branderi infection in a horse with granulomatous
mesenteric lymphadenitis’, Journal of Comparative Pathology, 168,
pp. 30–34. doi: 10.1016/j.jcpa.2019.03.003.
Simmonds, R. C.
(2017) ‘Chapter 4. Bioethics and animal use in programs of research,
teaching, and testing’, in Weichbrod, R. H.,
Thompson, G. A. and Norton, J. N. (eds.) Management of
Animal Care and Use Programs in Research, Education, and Testing. 2nd ed. Boca Raton: CRC
Press, pp. 35–62. doi:
10.1201/9781315152189-4.
Thorel, M. F., Huchzermeyer, H. F. A. K. and
Michel, A. L. (2001) ‘Mycobacterium avium
and Mycobacterium intracellulare infection in
mammals’, Revue Scientifique et Technique de
l’OIE, 20(1), pp. 204–218. doi: 10.20506/rst.20.1.1272.
Trčko, K., Plaznik, J. and Miljković, J. (2021) ‘Mycobacterium marinum hand infection masquerading as tinea manuum: A case report and
literature review’, Acta Dermatovenerologica Alpina Pannonica et Adriatica,
30(2), pp. 91–93. doi:
10.15570/actaapa.2021.23.
VRU (Verkhovna Rada Ukrainy) (2006) ‘Law of Ukraine No. 3447-IV of
Weese, J. S. and Gomez‐Nieto, D. (2016) ‘Mycobacterial infections in horses’,
Equine Veterinary Education, 28(11), pp. 623–624. doi: 10.1111/eve.12423.
Xin, L., Xu, L., Jin, J., Ren, X.,
Zhu, L., Cheng, J., Li, J., Wang, T. and Wang, N.
(2022). ‘Pathogenicity of Mycobacterium avium to guinea pigs’, Microbiology China,
49(12), pp. 5277–5286. doi:
10.1016/j.vetmic.2024.110250.
Zavgorodnii, A. I., Bilushko, V. V.,
Kalashnyk, M. V., Pozmogova, S. A.
and Kalashnyk, N. V. (2018)
‘Pseudo-allergic reactions to tuberculin in cattle’ [Psevdoalerhichni reaktsii na tuberkulin u velykoi rohatoi khudoby], Veterinary Biotechnology [Veterynarna
biotekhnolohiia], 32(2), pp. 176–184. doi: 10.31073/vet_biotech32(2)-20. [in
Ukrainian].
Zavgorodnii, A. I., Pozmogova, S. A.,
Kalashnyk, M. V., Paliy, A. P.,
Plyuta, L. V. and Palii, A. P.
(2021) ‘Etiological factors in triggering non-specific allergic reactions
to tuberculin in cattle’, Regulatory Mechanisms in Biosystems,
12(2), pp. 228–233. doi:
10.15421/022131.
Zavgorodniy, A. I., Bilushko, V. V.,
Pozmogova, S. A., Kalashnyk, M. V.,
Kalashnyk, N. V., Kiptenko, A. V.
and Steshenko, L. M. (2021)
‘Determination of the causes of allergic reactions to tuberculin in
cattle’ [Vyznachennia prychyn
alerhichnykh reaktsii na tuberkulin u velykoi rohatoi khudoby], Veterinary Medicine [Veterynarna
medycyna], 107, pp. 30–36. doi: 10.36016/VM-2021-107-5.
[in Ukrainian].
Zavgorodniy, A. I., Bilushko, V. V.,
Pozmogova, S. A., Kalashnyk, M. V.
and Busol, V. O. (2023) ‘Problems in
the diagnosis of Bovine tuberculosis’ [Problemy
diahnostyky tuberkulozu velykoi rohatoi khudoby], Veterinary Medicine [Veterynarna
medycyna], 109, pp. 15–18. doi: 10.36016/VM-2023-109-3.
[in Ukrainian].
Zhurilo, O. A., Barbova, A. I. and Sladkova, L. M.
(2020) ‘Mycobacterium avium as pathogen
of human mycobacteriosis [Mycobacterium avium — zbudnyk mikobakteriozu liudyny]’, Ukrainian
Pulmonology Journal [Ukrainskyi pulmonolohichnyi
zhurnal], 107(1), pp. 50–58. doi: 10.31215/2306-4927-2020-107-1-50-58.
[in Ukrainian].
