Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
11, Issue 4, November 2025, Pages 24–31
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
STUDY
OF THE BIOCHEMICAL PROPERTIES OF BORDETELLA
BRONCHISEPTICA CLINICAL ISOLATES
Hadzevych D. V.
National Scientific
Center ‘Institute of Experimental and Clinical Veterinary Medicine’,
Kharkiv, Ukraine, e-mail: d5195681@gmail.com
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PDF (print version)
Citation for print version: Hadzevych, D. V.
(2025) ‘Study of the biochemical
properties of Bordetella bronchiseptica clinical isolates’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 11(4), pp. 24–31.
Download
PDF (online version)
Citation for online version: Hadzevych, D. V.
(2025) ‘Study of the biochemical
properties of Bordetella bronchiseptica clinical isolates’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 11(4), pp. 24–31. DOI: 10.36016/JVMBBS-2025-11-4-4.
Summary. During the first half of 2025, we isolated
five isolates of Bordetella bronchiseptica from dogs with bordetellosis.
These isolates exhibited low enzymatic activity, which is characteristic of B. bronchiseptica. Specifically, they did not produce indole, liquefy gelatin, ferment sugars, or produce the
enzyme tyrosinase. However, they produced the enzyme
urease and exhibited catalase and oxidase activity. Additionally, they grew on
Simmons citrate agar and reduced nitrates to nitrites. Notably, they did not produce
hydrogen sulfide on trisaccharide agar with the
addition of iron. Our improved method for identifying and differentiating B. bronchiseptica isolates enabled us to significantly
simplify and accelerate the isolation of pure Bordetella
cultures by 2–3 days. Identification of clinical isolates of B. bronchiseptica took 3–4 days
Keywords: Bordetellosis, dogs,
identifying, differentiating, pure culture
References:
Bemis, D. A.
(1992) ‘Bordetella and mycoplasma
respiratory infections in dogs and cats’, Veterinary Clinics of North
America: Small Animal Practice, 22(5), pp. 1173–1186. doi: 10.1016/s0195-5616(92)50308-4.
Bemis, D. A., Shek, W. R.
and Clifford, C. B. (2003) ‘Bordetella bronchiseptica infection of rats and
mice’, Comparative Medicine, 53(1), pp. 11–20. Available at: https://aalas.kglmeridian.com/view/journals/72010023/53/1/article-p11.xml.
Chambers, J. K., Matsumoto, I.,
Shibahara, T., Haritani, M.,
Nakayama, H. and Uchida, K. (2019) ‘An outbreak of fatal Bordetella bronchiseptica bronchopneumonia in
puppies’, Journal of Comparative Pathology, 167,
pp. 41–45. doi:
10.1016/j.jcpa.2018.12.002.
Clements, J.,
McGrath, C. and McAllister, C. (2018) ‘Bordetella bronchiseptica pneumonia: Beware of the
dog!’, BMJ
Case Reports, 2018, p. bcr-2018-224588. doi: 10.1136/bcr-2018-224588.
Ellis, J. A.
(2015) ‘How well do vaccines for Bordetella bronchiseptica
work in dogs? A critical review of the literature
1977–2014’, The Veterinary Journal, 204(1),
pp. 5–16. doi:
10.1016/j.tvjl.2015.02.006.
Gadzevich, D. V. (2024) ‘Study of the
effectiveness of using a selective cephalexin component in a CCA nutrient medium for the detection of clinical isolates
of Bordetella bronchiseptica’
[Vyvchennia efektyvnosti zastosuvannia selektyvnoho komponentu tsefaleksyn u pozhyvnomu seredovyshchi KVA dlia vydilennia klinichnykh izoliativ Bordetella bronchiseptica],
Veterinary Medicine [Veterynarna medycyna], 110, рр. 81–87. doi: 10.36016/vm-2024-110-12. [in Ukrainian].
Gerlach, G., von Wintzingerode, F.,
Middendorf, B. and Gross, R. (2001)
‘Evolutionary trends in the genus Bordetella’,
Microbes and Infection, 3(1),
pp. 61–72. doi: 10.1016/s1286-4579(00)01353-8.
Gueirard, P.,
Weber, C., Le Coustumier, A. and Guiso, N.
(1995) ‘Human Bordetella bronchiseptica
infection related to contact with infected animals: Persistence of bacteria in
host’, Journal of Clinical Microbiology, 33(8), рр. 2002–2006. doi: 10.1128/jcm.33.8.2002-2006.1995.
Hadzevych, D. V.
(2024) ‘Bacteriological studies of probe swabs with nasopharyngeal
secretions from canines diagnosed with Bordetellosis’,
Journal for Veterinary Medicine, Biotechnology and Biosafety, 10(4), рр. 33–39. doi: 10.36016/jvmbbs-2024-10-4-6.
Harvill, E. T.,
Preston, A., Cotter, P. A., Allen, A. G., Maskell, D. J. and Miller, J. F. (2000)
‘Multiple roles for Bordetella lipopolysaccharide molecules during respiratory
tract infection’, Infection and Immunity, 68(12), рр. 6720–6728. doi: 10.1128/iai.68.12.6720-6728.2000.
Henderson, I. R.
and Nataro, J. P. (2001) ‘Virulence
functions of autotransporter proteins’, Infection
and Immunity, 69(3), рр. 1231–1243. doi:
10.1128/iai.69.3.1231-1243.2001.
Inatsuka, C. S.,
Julio, S. M. and Cotter, P. A. (2005) ‘Bordetella
filamentous hemagglutinin plays a critical role in
immunomodulation, suggesting a mechanism for host specificity’, Proceedings
of the National Academy of Sciences, 102(51), pp. 18578–18583. doi: 10.1073/pnas.0507910102.
Jacob-Dubuisson, F., Kehoe, B., Willery, E.,
Reveneau, N., Locht, C.
and Relman, D. A. (2000) ‘Molecular
characterization of Bordetella bronchiseptica
filamentous haemagglutinin and its secretion
machinery The GenBank accession numbers for the
sequences reported in this paper are AF111794, AF111796, AF111797 and AF111798.’, Microbiology, 146(5),
pp. 1211–1221. doi: 10.1099/00221287-146-5-1211.
Kadlec, K. and Schwarz, S. (2018)
‘Antimicrobial resistance in Bordetella bronchiseptica’, Microbiology Spectrum,
6(4), p. ARBA-0024-2017. doi: 10.1128/microbiolspec.arba-0024-2017.
Kameyama, H.,
Fujimoto, Y., Tomioka, Y.,
Yamamoto, S., Suyama, H., Inoue, H.,
Takahashi, E. and Ono, E. (2022) ‘Pathogenicity of Bordetella bronchiseptica
isolated from apparently healthy rabbits in guinea pig, rat, and mouse’, Journal
of Veterinary Medical Science, 84(4), pp. 574–581. doi: 10.1292/jvms.21-0494.
Korniienko, L. Ye.,
Busol, V. O., Nedosiekov, V. V.,
Ushkalov, V. O., Holovko, A. M.,
Korniienko, L. V. and Dombrovskyi, O. B.
(2008) Chronic Infectious Diseases of Animals [Khronichni
infektsiini khvoroby tvaryn]. Bila Tserkva: Bila Tserkva
National Agrarian University. Available at: https://rep.btsau.edu.ua/handle/BNAU/1294.
MHU (Ministry of
Health of Ukraine) (2005) Methodological
Guidelines for Microbiological Diagnostics of Pertussis and Parapertussis:
approved by the order of the Ministry of Health of Ukraine of 15 April
2005 No. 169’ [Metodychni vkazivky z mikrobiolohichnoi diahnostyky kashliuku ta parakashliuku: zatverdzheno nakazom Ministerstva okhorony zdorovia Ukrainy vid 15 kvitnia
2005 r. № 169]. Available at: https://mozdocs.kiev.ua/view.php?id=3910. [in Ukrainian].
MHU (Ministry of
Health of Ukraine) (2007) Methodological
Guidelines ‘Determination of the Sensitivity of Microorganisms to
Antibacterial Drugs’: approved by the order of the Ministry of Health of
Ukraine of 5 April 2007 No. 167’ [Metodychni
vkazivky “Vyznachennia
chutlyvosti mikroorhanizmiv
do antybakterialnykh preparativ”:
zatverdzheno nakazom Ministerstva okhorony zdorovia Ukrainy vid 5 kvitnia 2007 r. № 167]. Available at: https://mozdocs.kiev.ua/view.php?id=6958. [in Ukrainian].
Miguelena Chamorro, B.,
De Luca, K., Swaminathan, G., Longet, S., Mundt, E.
and Paul, S. (2023) ‘Bordetella bronchiseptica and Bordetella pertussis: Similarities and differences in infection, immuno-modulation, and vaccine considerations’, Clinical
Microbiology Reviews, 36(3),
p. e0016422. doi: 10.1128/cmr.00164-22.
Milanko, A. Ya., Gerilovich, P. P. and Dushkin, D. V.
(1995) ‘Bordetella bronchiseptica
infection in dogs’ [Bordetelleznaya infektsiya sobak], Proceeding of Scientific Conference of the
Kharkov Zooveterinary Institute [Materialy nauchnoj konferencii Harkovskogo zooveterinarnogo instituta], pp. 34–35. [in Russian].
Milanko, A. Ya., Kholodylo, Ye. V. and Dushkyn, D. V. (1995) ‘The role of Bordetella bronchiseptica
in the diagnosis of whooping cough-like diseases’ [Rol’
Bordetella bronchiseptica
v diagnostike koklyushepodobnykh
zabolevaniy], Proceedings
of the IV Congress of Parasitocenologists of
Ukraine [Materialy
IV s”ezda parazitotsenologov
Ukrainy], p. 14. [in
Russian].
Moore, J. E.,
Rendall, J. C. and Millar, B. C.
(2021a) ‘A doggy tale: Risk of zoonotic
infection with Bordetella bronchiseptica
for cystic fibrosis (CF) patients from live licenced
bacterial veterinary vaccines for cats and dogs’, Journal of Clinical
Pharmacy and Therapeutics, 47(2),
pp. 139–145. doi: 10.1111/jcpt.13492.
Moore, J. E.,
Rendall, J. C. and Millar, B. C.
(2021b) ‘Does Bordetella pertussis vaccine offer any cross-protection against Bordetella bronchiseptica?
Implications for pet owners with cystic fibrosis’, Journal
of Clinical Pharmacy and Therapeutics, 46(5), pp. 1194–1198. doi: 10.1111/jcpt.13350.
Mylanko, O. Ya. and Dushkin, D. V. (1996) ‘Properties of Bordetella bronchiseptica
cultures isolated from dogs’ [Vlastyvosti kultur Bordetella bronchiseptica vydilenykh vid
sobak], Proceeding
of Scientific Conference of the Sumy Agricultural Institute [Materialy naukovoi konferentsii Sumskoho Silskohospodarskoho Instytutu],
pp. 47–49. [in Ukrainian].
Schulz, B. S.,
Kurz, S., Weber, K., Balzer, H. J.
and Hartmann, K. (2014) ‘Detection of respiratory viruses and Bordetella bronchiseptica
in dogs with acute respiratory tract infections’, The Veterinary
Journal, 201(3), pp. 365–369. doi: 10.1016/j.tvjl.2014.04.019.
Taha-Abdelaziz, K., Bassel, L. L., Harness, M. L.,
Clark, M. E., Register, K. B. and Caswell, J. L.
(2016) ‘Cilia-associated bacteria in fatal Bordetella bronchiseptica pneumonia of dogs and
cats’, Journal of Veterinary Diagnostic Investigation, 28(4),
pp. 369–376. doi:
10.1177/1040638716646806.
Woods, P., Ordemann, K., Stanecki, C.,
Brown, J. and Uzodi, A. (2019) ‘Bordetella bronchiseptica
pneumonia in an adolescent: Case report and review of the pediatric
literature’, Clinical Pediatrics, 59(3), pp. 322–328. doi: 10.1177/0009922819897355.
