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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 9, Issue 3, September 2023, Pages 11–17

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF MORPHOLOGICAL, KARYOLOGICAL, BIOLOGICAL CHARACTERISTICS OF VIRUS-PRODUCING CELL LINES FLK-BLV (FLK‑POL, FLK‑71, FLK 50/100, FLK‑SBBL) DURING LONG-TERM STORAGE IN THE CRYOBANK OF THE NATIONAL COLLECTION OF THE NSC ‘IECVM’

Stegniy M. Yu., Stegniy B. T., Hrytsyna N. V.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: marina.stegniy@gmail.com

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Citation for print version: Stegniy, M. Yu., Stegniy, B. T., Hrytsyna, N. V. (2023) ‘Study of morphological, karyological, biological characteristics of virus-producing cell lines FLK-BLV (FLK‑POL, FLK‑71, FLK 50/100, FLK‑SBBL) during long-term storage in the cryobank of the National collection of the NSC ‘IECVM’’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 9(3), pp. 11–17.

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Citation for online version: Stegniy, M. Yu., Stegniy, B. T., Hrytsyna, N. V. (2023) ‘Study of morphological, karyological, biological characteristics of virus-producing cell lines FLK-BLV (FLK‑POL, FLK‑71, FLK 50/100, FLK‑SBBL) during long-term storage in the cryobank of the National collection of the NSC ‘IECVM’’, Journal for Veterinary Medicine, Biotechnology and Biosafety. [Online] 9(3), pp. 11–17. DOI: 10.36016/JVMBBS-2023-9-3-3.

Summary. Studies have shown that, morphologically, the virus-producing cell line (FLK 50/100) consists of epithelial-like cells with a perinuclear space around the nucleus, while the FLK‑71 line is represented by fibroblast-like cells with large nuclei. The karyology of FLK‑71 cell lines after long-term storage was characterized by an increase in the range of chromosome number fluctuations from 40 to 64 from the data sheet of these cells before freezing (from 42 to 56). The limits of chromosome number fluctuation in FLK 50/100 decreased and ranged from 40 to 68. The antigen-producing biological activity of the subline of the reinoculated FLK-BLV culture (FLK‑Pol) was preserved from passage 5 and further up to 22 passages (passage period) during the studied storage periods, namely from 55.5 months (FLK‑Pol) to 201 months of storage (FLK‑SBBL) and 206.5 months (FLK 50/100) in the conditions of the NSC ‘IECVM’ cryobank. However, for the strain FLK 50/100, up to 10 passages after thawing are required to fully recover the titers of its biological activity

Keywords: virus antigens, mitotic activity, immunodiffusion test

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