Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 1, March 2017, Pages 17–20
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DImETHYL SuLfOXIDE
AS THE STImuLANT ANTIGEN PRODuCING ACTIVITY Of THE CELL CuLTuRE fLK-BLV
Gorbatenko S. K. 1 ,
Stegniy M. Yu. 1 ,
Korneykov A. N. 1 , Myagkyh
N. V. 1 , Magats D. Yu. 1
, Gorbatenko V. P. 2
1 National Scientific Center ‘Institute of Experimental and
Clinical Veterinary Medicine’, Kharkiv,
Ukraine, admin@vet.kharkov.ua
2 Kharkiv State Zooveterinary Academy, Kharkiv,
Ukraine
Download
PDF (print version)
Citation for print version: Gorbatenko, S. K., Stegniy, M. Yu., Korneykov, A. N.,
Myagkyh, N. V., Magats, D.
Yu. and Gorbatenko, V. P. (2017)
‘Dimethyl sulfoxide as the stimulant antigen
producing activity of the cell culture FLK-BLV’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety, 3(1), pp. 17–20.
Download
PDF (online version)
Citation for online version: Gorbatenko, S. K., Stegniy, M. Yu., Korneykov, A. N.,
Myagkyh, N. V., Magats, D.
Yu. and Gorbatenko, V. P. (2017)
‘Dimethyl sulfoxide as the stimulant antigen
producing activity of the cell culture FLK-BLV’,
Journal for Veterinary Medicine,
Biotechnology and Biosafety. [Online] 3(1), pp. 17–20. Available at:
http://jvmbbs.kharkov.ua/archive/2017/volume3/issue1/oJVMBBS_2017031_017-020.pdf
Summary.
The article observes a capability of the dimethyl sulfoxide
(DMSO) use to stimulate the leukemia virus expression
and antigen producing activity. Adhesive and proliferative activities of
stimulated cell culture FLK-BLV were
assessed at each passage of cell growth. Antigen producing activity was
determined after the adaptation period in the 5–10 passages by RID. The
addition of 1.0% dimethyl sulfoxide (DMSO) positively effects on the cells monolayer within 10
passages of the experiment. The activity of the experimental antigen produced
on nutrient medium with the addition of the DMSO was
higher 1.75 times than in control group.
Keywords: leukosis, cattle,
cells culture, radial immunodiffusion, leukemia virus
References:
De Giuseppe, A., Feliziani, F., Rutili, D. and De Mia, G.M.
(2004) ‘Expression of the bovine leukemia virus envelope Glycoprotein (gp51) by recombinant Baculovirus
and its use in an enzyme-linked Immunosorbent
assay’, Clinical and Vaccine Immunology, 11(1), pp. 147–151.
http://dx.doi.org10.1128/cdli.11.1.147-151.2004
Dyakonov, L. P.
(ed.) (2009) Animal cells in culture
(methods and application in biotechnology) [Zhivotnaya kletka v kul’ture (metody i primenenie v biotekhnologii)]. Moscow: Sputnik+. ISBN
9785997302146. [in Russian]
Gilljam,
G., Siridewa, K. and Hammar,
L. (1994) ‘Purification of simian immunodeficiency virus, SIVMAC251, and of its external envelope glycoprotein, gp148’, Journal of Chromatography A, 675(1-2),
pp. 89–100. http://dx.doi.org10.1016/0021-9673(94)85263-4
Hammar,
L., Eriksson, S., Malm, K. and Morein,
B. (1989) ‘Concentration and purification of feline leukaemia
virus (FeLV) and its outer envelope protein gp70 by aqueous two-phase systems’, Journal of Virological Methods, 24(1-2), pp. 91–101. http://dx.doi.org10.1016/0166-0934(89)90011-6
Hammar,
L. and Gilljam, G. (1990) ‘Extraction of HIV-1
in aqueous two-phase systems to obtain a high yield of gp120’,
AIDS Research and Human Retroviruses, 6(12), pp. 1379–1388. http://dx.doi.org10.1089/aid.1990.6.1379
Hammar,
L., Merza, M., Malm, K.,
Eriksson, S. and Morein, B. (1989) ‘The use of
aqueous two-phase systems to concentrate and purify bovine leukemia virus outer
envelope protein gp51’, Biotechnology and
Applied Biochemistry, 11(3), pp. 296–306. http://dx.doi.org10.1111/j.1470-8744.1989.tb00062.x
OIE
(World Organisation for Animal Health) (2008)
‘Chapter 2.4.11. Enzootic Bovine Leukosis’, in Manual of diagnostic tests and vaccines for terrestrial animals. 6th ed. Vol. 2, pp. 729–738.
Available at: http://web.oie.int/eng/normes/MMANUAL/2008/pdf/2.04.11_EBL.pdf
OIE
(World Organisation for Animal Health) (2017) Enzootic
bovine leukosis. Available at: http://www.oie.int/wahis_2/public/wahid.php/Countryinformation/Countrytimelines
(Accessed: 17 January 2017)
State Committee of Veterinary Medicine of Ukraine (2007) Order
№ 21 from 21.12.2007 ‘On approval of
Guidance on prevention and rehabilitation of cattle from bovine leukemia’
[Nakaz № 21 vid 21.12.2007 ‘Pro zatverdzhennia Instruktsii z profilaktyky ta ozdorovlennia velykoi rohatoi khudoby vid leikozu’], Official Bulletin of Ukraine [Ofitsiinyi visnyk Ukrainy], 4, p. 39, art. 116. Available at: http://zakon.rada.gov.ua/laws/show/z0012-08. [in Ukrainian]
