Issue 1
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 1, March 2017, Pages 32–35
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
THE INTERLABORATORY
TESTING Of THE TEST-SYSTEm fOR DETECTION Of THE LumPY SKIN DISEASE VIRuS DNA uSING
REAL-TImE PCR ‘BOVI-DNA-TEST-LSD
VIRuS’
Nevolko O. M. 1 , Stegniy
B. T. 2 , Marushchak L. V. 1 , Sapachova M. A. 1 , Sushko
M. I. 1, Gerilovych A. P. 2
1 State Scientific and Research Institute
for Laboratory Diagnostics and Veterinary-Sanitary Expertise, Kyiv, Ukraine
2 National Scientific Center ‘Institute for Experimental and
Clinical Veterinary Medicine’, Kharkiv,
Ukraine, e-mail: antger2011@gmail.com
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PDF (print version)
Citation for print version: Nevolko, O. M., Stegniy, B. T., Marushchak, L. V.,
Sapachova, M. A., Sushko M.
I. and Gerilovych, A. P. (2017) ‘The interlaboratory testing of the test-system for detection of
the Lumpy skin disease virus DNA using real-time PCR ‘Bovi-DNA-test-LSD virus’’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 3(1), pp. 32–35.
Download
PDF (online version)
Citation for online version: Nevolko, O. M., Stegniy, B. T., Marushchak, L. V.,
Sapachova, M. A., Sushko M.
I. and Gerilovych, A. P. (2017) ‘The interlaboratory testing of the test-system for detection of
the Lumpy skin disease virus DNA using real-time PCR ‘Bovi-DNA-test-LSD virus’’, Journal for Veterinary Medicine, Biotechnology and Biosafety.
[Online] 3(1), pp. 32–35. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue1/oJVMBBS_2017031_032-035.pdf
Summary. Lumpy skin disease
(LSD, nodular dermatitis of cattle) — is the contagious poxviral disease of cattle. It characterized with severe
losses and different ranges of mortality and morbidity. The disease is endemic
in many Asian and African countries. National Scientific Center
‘Institute for Experimental and Clinical Veterinary Medicine’ in
collaboration with State Scientific and Research Institute for Laboratory
Diagnostics and Veterinary-Sanitary Expertise developed the in-house PCR-based protocol for LSDV
detection that requires fast implementation. The evaluation of diagnostics kit has been performed under OIE
requirements with determination of the sensitivity, specificity, repeatability
and domain-specificity. Detection kit is recommended
for practical application.
Keywords: lumpy skin
disease, diagnostics, real-time PCR, test system, cattle
References:
AHAW (EFSA Panel on Animal Health and Welfare)
(2015) ‘Scientific opinion on lumpy skin disease’, EFSA Journal, 13(1), p. 3986. http://dx.doi.org10.2903/j.efsa.2015.3986
Al‑Salihi, L.
(2014) ‘Lumpy Skin disease: Review of literature’, Mirror of Research in Veterinary Sciences
and Animals, 3(3), pp. 6–23. Available at: http://mrvsa.com/upload/3-3-2-2014%20Lumpy%20Skin%20disease%20%20Review%20of%20literature.pdf
Coetzer, J. A. W. (2004) ‘Lumpy skin disease’, in Coetzer, J. A. W. and
Tustin, R. C. (eds.) Infectious diseases of livestock. 2nd ed. Cape Town, South Africa: Oxford
University Press, pp. 1268–1276. ISBN 9780195761719
OIE (World Organisation
for Animal Health) (2016) ‘Chapter 2.4.13. Lumpy skin
disease’, in: Manual of diagnostic tests and vaccines for terrestrial
animals (mammals, birds and bees). Paris: OIE. Available
at: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.13_LSD.pdf
Stegniy, B. T. and Gerilovych, A. P.
(eds.) (2014) Molecular genetic methods of diagnostics in veterinary
medicine and biotechnology: A tutorial [Molekuliarno-henetychni
metody diahnostyky u veterynarnii medytsyni ta biotekhnolohii: navchalnyi posibnyk]. Kyiv: ST Druk.
ISBN 9789662717143. [in Ukrainian]
