Issue 3
Journal for Veterinary Medicine, Biotechnology and Biosafety
Volume
3, Issue 3, September 2017, Pages 10–17
ISSN 2411-3174 (print version) ISSN 2411-0388
(online version)
DEVELOPMENT OF PCR TEST SYSTEMS FOR SPECIES DIFFERENTIATION OF CHLAMYDIAL
AGENTS IN BIRDS
Кsyonz І. М. 1,
Korniyenko M. V. 1
1 Institute of Pig Breeding and Agroindustrial
Production of the National Academy of Agrarian Sciences of Ukraine, Poltava,
Ukraine, e-mail: igor.ksyonz@ukr.net
2 National Scientific Center ‘Institute of Experimental and Clinical
Veterinary Medicine’, Kharkiv, Ukraine, e-mail:
marina-korniienko@mail.ru
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PDF (print version)
Citation for print version: Кsyonz,
І. М. and Korniyenko, M. V. (2017)
‘Development of PCR test systems for species
differentiation of chlamydial agents in birds’, Journal for Veterinary Medicine, Biotechnology and
Biosafety, 3(3), pp. 10–17.
Download
PDF (online version)
Citation for online version: Кsyonz,
І. М. and Korniyenko, M. V. (2017)
‘Development of PCR test systems for species
differentiation of chlamydial agents in birds’, Journal for Veterinary Medicine, Biotechnology and
Biosafety. [Online] 3(3), pp. 10–17. Available at: http://jvmbbs.kharkov.ua/archive/2017/volume3/issue3/oJVMBBS_2017033_010-017.pdf
Summary. According to the modern classification
adopted by the 2nd European Meeting on Animal Chlamydioses
and Zoonotic Implications (EMAC‑2), the genus Chlamydia contains three species of bird
chlamydial agents — C. avium, C. gallinacea and C. psittaci. Three PCR test
systems have been developed for indication and species
differentiation of these bacteria. The bases for the developed diagnostics are
three pairs of oligonucleotide primers designed and synthesized to flank the
DNA fragments specific for each of the three bacteria species of the Chlamydia genus, which are pathogenic
for birds. Analytical specificity of the developed PCR
test systems was confirmed by the results of amplification of 18 biological
materials samples, among them 13 being Chlamydia-containing,
one of them contained C. avium, one — C. gallinacea, and 11 — C. psittaci.
In addition Leptospira and Babesia DNA
samples were tested. The developed PCR test systems
for indication and species differentiation of bird Chlamydiosis
agents permit to reliably study certain aspects of
chlamydial infection.
Keywords: Chlamydioses, birds, МОМР, PCR
test system, species differentiation, Chlamydia avium, Chlamydia gallinacea, Chlamydia psittaci
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