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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 11, Issue 4, November 2025, Pages 37–41

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF THE BACTERICIDAL ACTIVITY OF THE ‘DEZV ULTRA’ DISINFECTANT AGAINST MYCOBACTERIA

Zavgorodniy A. I., Pozmogova S. A., Paliy A. P., Bilushko V. V., Ushkalov A. V.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: andrii.i.zavgorodnii@gmail.com

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Citation for print version: Zavgorodniy, A. I., Pozmogova, S. A., Paliy, A. P., Bilushko, V. V. and Ushkalov, A. V. (2025) ‘Study of the bactericidal activity of the ‘DezV Ultra’ disinfectant against mycobacteria’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 11(4), pp. 37–41.

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Citation for online version: Zavgorodniy, A. I., Pozmogova, S. A., Paliy, A. P., Bilushko, V. V. and Ushkalov, A. V. (2025) ‘Study of the bactericidal activity of the ‘DezV Ultra’ disinfectant against mycobacteria’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 11(4), pp. 37–41. DOI: 10.36016/JVMBBS-2025-11-4-6.

Summary. Timely and effective disinfection of livestock facilities and the environment is essential for maintaining bovine tuberculosis control. Currently, a significant number of disinfectants are used for disinfection, but not all of them have a bactericidal effect against tuberculosis pathogens and atypical mycobacteria at concentrations and exposures recommended by developers. The introduction of new disinfectants into veterinary practice is not possible without first determining their biocidal properties against zoonotic pathogens, including pathogenic and atypical mycobacteria, in the laboratory. The bactericidal activity of the disinfectant ‘DezV Ultra’ was determined using the suspension method and on test objects using test cultures of mycobacteria (M. bovis, M. avium, M. fortuitum). The quality of disinfection was tested on laboratory animals. The study was conducted at the Laboratory of Tuberculosis of the National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’. According to the results of the studies, the disinfectant ‘DezV Ultra’ was found to destroy the tuberculosis pathogens M. bovis and M. avium on test objects with a biological load at concentrations of 2.0% and 3.0% with exposures of 24 h and 3 h, respectively. The bactericidal effect of ‘DezV Ultra’ against M. fortuitum in a suspension application was observed at a concentration of 1.0% for 24 h, 2.0% for 5 h, and 3.0% for 3 h. Conclusions: The disinfectant ‘DezV Ultra’ is recommended for the preventive and mandatory disinfection of premises in agricultural enterprises, regardless of whether they are considered safe or high-risk for bovine or poultry tuberculosis

Keywords: Mycobacterium, disinfection, glutaraldehyde, test objects, guinea pigs

References:

Milián-Suazo, F., González-Ruiz, S., Contreras-Magallanes, Y. G., Sosa-Gallegos, S. L., Bárcenas-Reyes, I., Cantó-Alarcón, G. J. and Rodríguez-Hernández, E. (2022) ‘Vaccination strategies in a potential use of the vaccine against Bovine tuberculosis in infected herds’, Animals, 12(23), p. 3377. doi: 10.3390/ani12233377.

Paliy, A. P., Stegniy B. T. and Vedmid O. V. (2016) ‘Disinfectants for Animal tuberculosis’ [Dezinfikuiuchi preparaty pry tuberkulozi tvaryn], Veterinary Medicine [Veterynarna medytsyna], 102, pp. 172‒175. Available at: https://jvm.kharkov.ua/sbornik/102/3_47.pdf. [in Ukrainian].

Paliy, A. P., Zavgorodniy, A. I., Stegniy, B. T. and Palii, A. P. (2020) Scientific and Methodological Grounds for Controlling the Development and Use of Disinfectants [Naukovo-metodychni osnovy kontroliu rozrobky ta zastosuvannia zasobiv dezinfektsii]. Kharkiv: Miskdruk. doi: 10.36016/VB-2020-1. [in Ukrainian].

PHCMOHU (Public Health Center of the Ministry of Health of Ukraine). (2025). Tuberculosis Monitoring in Ukraine and Regions [Monitorynh tuberkulozu v Ukraini ta rehionakh]. Available at: https://npsi.phc.org.ua/TB_Monitoring. [in Ukrainian].

Van Emden, H. F. (2019) Statistics for Terrified Biologists. 2^nd ed. Hoboken, NJ: John Wiley & Sons. ISBN 9781119563679.

WHO (World Health Organization). (2024) Global Tuberculosis Report 2024. Geneva: WHO. Available at: https://iris.who.int/server/api/core/bitstreams/7292c91e-ffb0-4cef-ac39-0200f06961ea/content.

WOAH (World Organisation for Animal Health). (2020) Meeting of the AD HOC Group on Alternative Strategies for the Control and Elimination of Mycobacterium tuberculosis complex Infection in Livestock. Paris: WOAH. Available at: https://www.woah.org/app/uploads/2023/03/a-ahg-alternativestrategies-m-tuberculosis-approved-1.pdf.

WOAH (World Organisation for Animal Health). (2023) ‘Chapter 8.12. Infection with Mycobacterium tuberculosis complex’, in Terrestrial Animal Health Code. 31^st ed. [chapter most recent update adopted in 2017]. Paris: WOAH. Available at: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahc/2023/chapitre_bovine_tuberculosis.pdf.

WOAH (World Organisation for Animal Health). (2024) Guidelines for the Control of Mycobacterium tuberculosis complex in Livestock. Beyond Test and Slaughter. Paris: WOAH. doi: 10.20506/woah.3538.

WOAH (World Organisation for Animal Health). (2025) ‘Mammalian Tuberculosis’. Available at: https://www.woah.org/en/disease/mammalian-tuberculosis/.

Zavgorodniy, A. I., Kalashnyk, N. V., Kochmarskyi, V. A., Busol, V. O., Paliy, A. P., Tykhonov, P. M. and Horzheiev, V. M. (2007) Determination of Bactericidal Properties of Disinfectants, Disinfection and Control of Its Quality in Tuberculosis of Farm Animals: Methodological Recommendations [Vyznachennia bakterytsydnykh vlastyvostei dezinfikuiuchykh zasobiv, provedennia dezinfektsii ta kontrol yii yakosti pry tuberkulozi silskohospodarskykh tvaryn: metodychni rekomendatsii]. Kharkiv. [in Ukrainian].

Zavgorodniy A. I., Stegniy B. T., Paliy A. P., Horzheiev, V. M. and Smirnov, A. M. (2013) Scientific and Practical Aspects of Disinfection in Veterinary Medicine’ [Naukovi ta praktychni aspekty dezinfektsii u veterynarnii medytsyni]. Kharkiv: FOP Brovin O. V. ISBN 9789662445596. [in Ukrainian].