Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 12, Issue 2, May 2026, Pages 36–40

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

STUDY OF THE BACTERICIDAL PROPERTIES OF AN OXYGEN-CONTAINING DISINFECTANT AGAINST MYCOBACTERIA

Zavgorodniy A. I. 1, Ushkalov A. V. 1, Bilushko V. V. 1, Pozmogova S. A. 1, Matviienko O. V. 2

1 National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: vetdocman@gmailcom

2 State Scientific Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine

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Citation for print version: Zavgorodniy, A. I., Ushkalov, A. V., Bilushko, V. V., Pozmogova, S. A. and Matviienko, O. V. (2026) ‘Study of the bactericidal properties of an oxygen-containing disinfectant against mycobacteria’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 12(2), pp. 36–40.

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Citation for online version: Zavgorodniy, A. I., Ushkalov, A. V., Bilushko, V. V., Pozmogova, S. A. and Matviienko, O. V. (2026) ‘Study of the bactericidal properties of an oxygen-containing disinfectant against mycobacteria’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 12(2), pp. 36–40. DOI: 10.36016/JVMBBS-2026-12-2-5.

Summary. This study aimed to determine the bactericidal properties of an oxygen-based disinfectant against mycobacteria and evaluate its effectiveness in decontaminating production surfaces. The study material was the universal, oxygen-containing disinfectant ‘Famidez Sanoksil 100’, which contains hydrogen peroxide, silver nitrate, and phosphoric acid. The preparation’s bactericidal activity was assessed against a Mycobacterium phlei test culture using the suspension method at concentrations of 0.5%, 1.0%, 2.0%, and 3.0%, under various exposure conditions. Additionally, tuberculocidal properties were determined on test objects (batiste, wood, and tile) contaminated with M. bovis culture. The decontamination effectiveness of the test objects was confirmed by a biological study on laboratory animals (guinea pigs), which included an intradermal tuberculin test as well as pathological and bacteriological examinations. The bactericidal activity of the disinfectant was found to be directly dependent on the concentration of the working solution and the exposure time. Destruction of the M. phlei test culture was observed after 48 h at a concentration of 1.0%, whereas a 2.0% solution of the disinfectant provided a tuberculocidal effect only after 24–48 h. The 3.0% solution was the most effective, ensuring complete inactivation of mycobacteria after 5 h, 24 h, and 48 h of contact. After treatment with a 3.0% solution of the preparation, no mycobacterial growth was detected on test objects contaminated with the M. bovis culture (after exposure for 5 h, 24 h, and 48 h). In the biological experiment, laboratory animals in the experimental groups did not react to the tuberculin injection, and no mycobacterial cultures were isolated from their biomaterial. These results indicate the disinfectant’s pronounced tuberculocidal properties and confirm its effectiveness in decontaminating mycobacterium-contaminated objects

Keywords: disinfection, Mycobacterium bovis, atypical mecobacteria, test objects, guinea pigs

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