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Journal for Veterinary Medicine, Biotechnology and Biosafety

Volume 12, Issue 3, September 2026, Pages 18–28

ISSN 2411-3174 (print version) ISSN 2411-0388 (online version)

IMPROVEMENT OF THE METHOD FOR THE BACTERIOLOGICAL ISOLATION OF BORDETELLA BRONCHISEPTICA FROM NASOPHARYNGEAL SWABS OF DOGS

Hadzevych D. V., Paliy A. P., Hadzevych О. V., Kovalenko L. V., Yurko P. S.

National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’, Kharkiv, Ukraine, e-mail: d5195681@gmail.com

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Citation for print version: Hadzevych, D. V., Paliy, A. P., Hadzevych, О. V., Kovalenko, L. V. and Yurko, P. S. (2026) ‘Improvement of the method for the bacteriological isolation of Bordetella bronchiseptica from nasopharyngeal swabs of dogs’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 12(3), pp. 18–28.

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Citation for online version: Hadzevych, D. V., Paliy, A. P., Hadzevych, О. V., Kovalenko, L. V. and Yurko, P. S. (2026) ‘Improvement of the method for the bacteriological isolation of Bordetella bronchiseptica from nasopharyngeal swabs of dogs’, Journal for Veterinary Medicine, Biotechnology and Biosafety, 12(3), pp. 18–28. DOI: 10.36016/JVMBBS-2026-12-3-3.

Summary. This study aimed to develop a selective medium for the preliminary enrichment of clinical material and to improve the efficiency of the bacteriological isolation of Bordetella bronchiseptica from nasopharyngeal swabs collected from dogs. The study was conducted from 2023 to 2025. The study material consisted of 63 nasopharyngeal swabs from dogs with clinical signs of respiratory disease, of which 21 had an acute course and 42 had a chronic course. To choose selective components, the reference strain B. bronchiseptica UNCSM‑110 and 14 clinical isolates of the pathogen were used. The study used bacteriological, biochemical, spectrophotometric, and molecular genetic methods. It was found that the most promising selective components were cephalexin (40 mg/L), gentian violet (0.0025 g/L), and basic fuchsin (0.005 g/L), which effectively suppressed the accompanying nasopharyngeal microflora while allowing B. bronchiseptica to grow. Based on these, three variants of selective media were developed for the preliminary enrichment of clinical material. B. bronchiseptica DNA was detected by PCR in 47 of 63 samples (74.6%). In direct bacteriological culture, the pathogen was isolated in 8 cases (12.7%), whereas after selective enrichment, it was isolated in 14 cases (22.2%), a statistically significant increase compared with direct culture (p < 0.05). Maximum efficiency of bacterial isolation was observed after 6 hours of selective enrichment. The isolation rate of B. bronchiseptica following selective enrichment in dogs with acute respiratory disease was 42.9%, and in those with chronic respiratory disease, it was 11.9% (OR = 5.55; 95% CI 1.56–19.73). The use of the developed selective enrichment method reduces the negative impact of concomitant microflora and increases the efficiency of bacteriological isolation of viable B. bronchiseptica cultures from clinical samples obtained from dogs

Keywords: bordetellosis, selective medium, selective enrichment, Regan–Lowe agar

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